人参皂苷Rg1对小鼠全脑缺血致白质纤维损伤的保护作用及其机制

Protective effect of ginsenoside Rg1 on injury of white matter after global cerebral ischemia and its mechanism

目的:研究人参皂苷Rg1对小鼠全脑缺血致白质纤维损伤的影响,阐明人参皂苷Rg1对小鼠全脑缺血的保护作用及其机制。方法:雄性ICR小鼠制备缺血动物模型前灌胃人参皂苷50mg·kg-1,给药7d。末次给药1h后闭塞双侧的颈总动脉60min。实验共分为4个组:假手术组、缺血组、药物对照组及药物保护组。采用免疫组织化学方法检测全脑缺血后海马齿状回区白质纤维损伤情况;采用Western blotting方法检测MDA蛋白的表达。结果:与假手术组比较,缺血组MBP阳性细胞数量减少(P<0.05);与缺血组比较,药物保护组MBP阳性细胞数量增加(P<0.05)。与假手术组比较,缺血组MDA蛋白表达量增加(P<0.05);与缺血组比较,药物保护组MDA蛋白表达量减少(P<0.05)。结论:人参皂苷Rg1能够减轻小鼠全脑缺血所致的白质纤维损伤,其可能与减少MDA蛋白的表达有关。

Objective To study the influence of ginsenoside Rg1on injury of white matter and illuminate the protective effect of ginsenoside Rg1and its mechanism after cerebral ischemia.Methods Male ICR mice were pre-treated with ginsenoside Rg1（50mg·kg-1）by intragastric（i.g.）injection once a day for 7days.One hour after the last injection bilateral common carotid arteries were occluded for 60min.The mice were divided into four groups：sham group （Sal-Sham）,drug control group（Gs-Sham）,ischemia group（Sal-Isc）and drug-protective group（Gs-Isc）.The injury of white matter in dentate gyrus of hippocampus after global cerebral ischemia was detected with immunohistochemical staining.The MDA protein expression after cerebral ischemia was determined with Western blotting method.Results Compared with sham group,the MBP positive cells in ischemia group were decreased （P〈0.05）;compared with ischemia group,the MBP positive cells in drug-protective group were increased （P〈0.05）;compared with sham group,the MDA protein in ischemia group was increased （P〈0.05）; compared with ischemia group,the MDA protein in drug-protective group was decreased （P 〈0.05）. Conclusion Ginsenoside Rg1can attenuate the injury of white matter after cerebral ischemia and its mechanism might be associated with decreasing the MDA protein expression.