不同生长期的叶下珠中槲皮素含量比较

Determination of Quercetin in Phyllanthus Urinaria L.from Different Growth Periods

目的采用高效液相色谱法测定不同生长期的叶下珠中槲皮素的含量。方法色谱柱为Zorbax SB-C18柱(250mm×4.6mm,5.0μm),流动相为乙腈-0.2%磷酸(30:70),流速为1mL/min,紫外检测波长为370nm。结果槲皮素进样量在0.05～0.40μg范围内与峰面积具有良好的线性关系,r=0.9999(n=8),平均回收率为102.54%,RSD=1.74%(n=6)。结论 9月至10月采收的叶下珠中槲皮素的含量最高。

Objective To develop an HPLC method to determine the contents of quercetin in Phyllanthus urinaria L.from different growth periods.Methods The extracts were seperated on Zorbax SB-C18 column（250 mm × 4.6 mm,5.0 μm） with acetonitrile-0.2% phosphoric acid（30：70） as the mobile phase.The flow rate was 1 mL /min.The detection wavelength was at 370 nm.Results The method was linear within the range of 0.05-0.40 μg（r = 0.999 9）.The average recovery rate was 102.54%,RSD was 1.74%（n = 6）.Conclusion Phyllanthus urinaria L.grown in September and October is found to contain the highest contents of quercetin.