Feridex标记胎盘来源的间充质干细胞分化为神经干细胞的实验研究

Experimental Study of Feridex Labeled Placenta-Derived Mesenchymal Stem Cells Differentiating into Neural Stem Cells

目的分离胎盘组织来源的间充质干细胞并诱导分化为神经干细胞,以期找到能成功标记神经干细胞的方法。方法将胎盘组织剪碎后消化、培养,加入神经干细胞诱导因子10ng/mL表皮生长因子(EGF)+10ng/mL重组人碱性成纤维生长因子(bFGF)+DMEM/F12,并分别添加相应浓度的血清,预诱导3d后加入DMEM/F12+0.1μmol/L全反式维甲酸(RA),10ng/mL胶质细胞系源性神经营养因子(GDNF)+10ng/mL脑源性神经营养因子(BDNF)进行正式诱导7d。结果诱导10d后用Feridex标记诱导后的细胞,普鲁士蓝染色显示90%以上的干细胞胞质内出现细小的蓝色铁颗粒,Nestin染色显示阳性。结论胎盘来源的间充质干细胞能成功诱导为神经干细胞,且Feridex可成功标记诱导后的细胞。

Objective To isolate the human placenta-derived mesenchymal stem cells from the placenta tissues and to induce them into the neural stem cells for hoping to find one method successfully labelling the neural stem cells.Methods After being cut into pieces,the placenta tissues were digested,cultured,and added with the neural stem cells inducing factor 10 ng /mL EGF ＋ 10 ng /mL bFGF ＋ DMEM /F12 and corresponding concentrations of serum to preinduce for 3 d.Then DMEM /F12 ＋ 0.1 μmol /L RA,10 ng /mL GDNF ＋ 10 ng /mL BDNF were added to formally induce for 7 d.Results After 10 d inducing,prussian blue staining showed that in cytoplasma of 90% of stem cells marked by Feridex,blue iron tiny particle could be seen with positive Nestin staining.Conclusion The placenta-derived mesenchymal stem cells can be differentiated into the neural stem cells,and Freidex can successfully label the induced cells.