高效液相色谱法测定壮肾安神片中淫羊藿苷含量

Determination of Icraiin in Zhuangshen Anshen Tablets by HPLC

目的建立测定壮肾安神片中淫羊藿苷含量的高效液相色谱法。方法采用Shimadzu C18色谱柱(250mm×4.6mm,5μm),以乙腈-水(30:70)为流动相,流速1.0mL/min,检测波长270nm。结果淫羊藿苷进样量在8.56～136.96ng范围内与峰面积线性关系良好(r=0.9999),平均回收率为98.60%,RSD=2.57%(n=6)。结论高效液相色谱法简便、灵敏、准确,可用于壮肾安神片的质量控制。

Objective To establish the content determination method of icraiin in Zhuangshen Anshen Tablets by HPLC.Methods Shimadzu C18 column（250 mm × 4.6 mm,5 μm） was adopted,the mobile phase was acetonitrile-water（V /V,30：70）,the detection wavelength at 270 nm,the flow rate at 1.0 mL /min.Results The standard curve was linear over the range of 8.56-136.96 ng（r = 0.999 9）,and the average recovery rate was 98.60% with RSD of 2.57%（n = 6）.Conclusion The method is simple,sensitive and accurate,which is available for the quality control of Zhuangshen Anshen Tablets.