鸡IGF-Ⅰ基因单核苷酸多态性及其与屠体性状关系的研究

SNP of IGF-Ⅰ Gene and its Association with Carcass Traits in Chicken

本研究采用PCR-RFLP技术,对438只黄羽矮小型肉鸡的胰岛素样生长因子-Ⅰ基因(IGF-Ⅰ)的5′端进行遗传多态性研究,发现该区域的PCR扩增产物经HinfⅠ酶切后出现GG、GT和TT 3种基因型。经χ2检验,群体在该位点上处于Hardy-Weinberg平衡状态(P>0.05)。方差分析结果显示,性别对9个性状(活体重、屠体重、皮脂厚、半净膛、全净膛、胸肌重、腿肌重、腹脂重、腹脂率)有极显著影响(P<0.01),对肌间脂肪含量有显著影响(P<0.05)。家系效应在8个性状上(活体重、屠体重、半净膛、全净膛、胸肌重、腿肌重、腹脂重、腹脂率)达到极显著水平(P<0.01);而基因型效应只在腹脂率上达到显著水平(P<0.05),腹脂重没有达到显著水平(P=0.0669)。不同基因型的最小二乘均值间多重比较结果发现,TT型的腹脂重和腹脂率均显著低于GT型(P<0.05),而GG型和GT、TT型的差异不显著(P>0.05)。

In this study,the genetic polymorphism of insulin-like growth factorⅠ（IGF-Ⅰ）gene of 5′region in 438 yellow feather dwarf broilers was detected by PCR-RFLP method.PCR products treated by HinfⅠappeared GG,GT and TT genotypes.χ2 test showed the population was in Hardy-Weinberg equilibrium in this locus（P〈0.05）.Variance analysis indicated gender had extremely significant effect（P〈0.01）on 9traits（body weight and carcass weight,cutaneous fat thickness,halfeviscerated weight,eviscerated weight,breast muscle weight,drumstick weight,abdominal fate weight）and significant effect（P 0.05）on intramuscular fat,that family affected 8traits extremely significantly（P〈0.01）,while that genotype effect was suggestively associated with abdominal fat rate（P〈0.05）,approximately suggestively associated with abdominal fat weight（P =0.0669）.Multiple comparison of different genotypes’least square mean displayed abdominal fat weight and abdominal fat rate of genotype TT were significantly lower than that of genotype GT（P〈0.05）,while that of genotype GG was no different significantly from genotype GT and TT（P〈0.05）.