芝麻叶多糖的提取及抗氧化活性研究

Studies on Extraction and Antioxidant Activity of Polysaccharides from Sesame Leaves

目的:探讨芝麻叶水溶性多糖的提取条件及其抗氧化活性。方法:采用三因素三水平正交试验设计,研究提取温度、提取时间和料液比等因素对芝麻叶水溶性多糖提取率的影响,并对芝麻叶水溶性多糖清除O2-.、.OH、DPPH.自由基的能力进行研究。结果:芝麻叶水溶性多糖的提取条件是:料液比1∶10,提取温度75℃,提取时间45 min。抗氧化试验结果表明,芝麻叶水溶性多糖具有较好的抗氧化活性,其清除O2-.、.OH、DPPH.的IC50值分别为0.0250、0.2031、2.0510 mg/mL。芝麻叶水溶性多糖是一种效果好、安全性高的新型天然抗氧化物质,值得深入研究其生理功能并开发功能产品。结论:获得芝麻叶水溶性多糖的最佳提取条件,在该条件下芝麻叶多糖的得率为4.32%。芝麻叶水溶性多糖具有较明显的体外抗氧化能力。

Objective：The extraction condition and antioxidation capability of polysaccharides from Sesame Leaves were studied. Methods： The effect of each factor such as temperature, time, and ratio of solid-liquid on the extraction rate of the water-soluble polysaccharides from Sesame Leaves was investigated using the methods of single factor test and orthogonal design test. The antioxidation ability of water-soluble polysaccharide from Sesame Leaves were evaluated by scavenging effects to DPPH radical, hydroxyl radical, and Superoxide radical. Results： The results showed the highest extraction rate of polysaccharides from Sesame Leaves could be obtained with a ratio of solid to liquid as 1：10, 75 ℃ for 45 min, and 3 times of extraction. The half inhibit concentrations （IC50） toO2-·、·OH、DPPH· were 0.0250, 0.2051 and 2.0510 mg/mL respectively. The water-soluble polysaccharides are a potential product of natural, safe, and effective antioxidant. Conclusion： The best condition for the extraction of polysaccharides from Sesame Leaves were obtained with the yield of extraction 4.52%. The polysaccharides possess a strong antioxidation capability in vitro.