510.6nm激光对脐血造血细胞的增殖作用

510.6 nm Laser Effect on the Proliferation of Hematopoietic Cells in Human Umbilical Cord Blood

介绍了５１０．６ｎｍ激光对造血细胞的刺激作用，促使颗粒－巨噬细胞集落形成单位（ＧＭ－ＣＦＵｃ）增多。从人脐带血中分离制备的有核细胞中，加入集落刺激因子（ＣＳＦ）后进行体外培养，形成的ＧＭ－ＣＦＵｃ为１８．６±１２．６／１０４；如果加入的ＣＳＦ是经激光照射后的细胞制成的，其ＧＭ－ＣＦＵｃ为４０．５±２０．２／１０４；如果先用激光照射脐带血的有核细胞，在培养时加入的ＣＳＦ是未经激光照射后的细胞制成的，也有增殖效应，ＧＭ－ＣＦＵｃ为３６．５±１８．５／１０４；如果脐带血中的有核细胞和制备ＣＳＦ的细胞都用激光照射，发现ＧＭ－ＣＦＵｃ明显增多，其值为５７．４±４１．２／１０４。

Effect of a 510.6 nm laser on the proliferation of hematopoietic cells in human umbilical cord blood was studied. Mononuclear hematopoietic cells were prepared from human umbilical cord blood, and cultured with CSF in vitro, the GM CFUc was 18.6±12.6/10 4. If the mononuclear cells were cultured with CSF which was prepared by laser irradiated cells, the GM CFUc was 40.5±20.2/10 4. If the mononuclear cells were irradiated by laser and then cultured with CSF, the GM CFUc was 36.5±18.5/10 4. If both the mononuclear cells and the cells for CSF preparation were irradiated by laser, the GM CFUc was increased significantly, it was 57.4±41.2/10 4.