预防性抗氟剂-I对鼠骨5种微量元素含量的影响

Effect of Preventive Anti-Fluorine Agent on 5 Trace Elements in Bone of Rats

目的探讨预防性抗氟剂-I对锌、钙、镁、铁、铜在大鼠骨骼中含量的影响。方法通过染氟大鼠亚慢性试验,应用火焰原子吸收光谱法测定染氟大鼠大腿骨中锌、钙、镁、铁、铜的含量,分析元素含量的变化。结果在染氟组与拮抗组的比较中,骨锌在高、中、低剂量拮抗组中的含量均比对应的染氟组高,且在中剂量组差异有统计学意义;骨钙在拮抗组中含量高于对应剂量的染氟组,且在中、低剂量组中差异有统计学意义;骨镁在中、低剂量拮抗组中含量显著高于对应剂量的染氟组;骨铁在中剂量的拮抗组中含量显著低于对应剂量的染氟组;骨铜在低剂量的拮抗组中含量显著低于对应剂量的染氟组。在拮抗组与对照组比较中,拮抗组中的骨锌含量均高与对照组,且高、中剂量组有统计学意义;骨钙含量在高、中、低剂量拮抗组显著高于对照组;拮抗组中的骨镁含量均高于对照组,且高、中剂量组差异有统计学意义;拮抗组中的骨铁含量均低于对照组,且中、低剂量组差异有统计学意义;骨铜含量在中、低拮抗组低于对照组。结论抗氟剂使锌、钙、镁3种元素在骨中的含量升高,而对铁、铜在骨中含量的影响不明显。

[Objective] To investigate the effect of anti-fluorine agent on the levels of Zn, Ca, Mg, Fe, Cu in rats＇ bone.[Methods]Through subchronic test in rats exposed to fluoride. Flame atomic absorption spectrometry was adopted to determine the Zn, Ca, Mg, Fe, Cu levels in the thighbone, and the level changes were analyzed.[Results]Comparing fluorine agent group with anti-fluorine agent group, the level of bone Zn in anti-fluorine agent groups of high, medium and low doses were higher than those of the corresponding fluorine agent groups, with a significance in medium dose group. The levels of bone Ca in anti-fluorine groups were higher than those of the corresponding dose groups with significance in medium and low dose groups. In medium and low dose anti-fluorine agent group, the level of bone Mg was higher than that in corresponding dose groups with significance. The level of bone Fe in medium dose anti-fluorine agent group was lower than that in corresponding fluorine agent group with significance. The level of bone Cu in low dose anti-fluorine agent group was lower than that in fluorine agent group. Comparing fluorine agent group with control group, the levels of bone Zn of anti-fluorine agent groups were higher than those in fluorine agent groups with significance in high and medium dose groups. The levels of bone Ca in medium and low dose group were higher than those in control group. The levels of bone Mg in anti-fluorine agent groups were all higher than those in control group with significance in high and medium dose groups. The levels of bone Fe in anti-fluorine agent groups were all lower than those in control groups with significance in medium and low dose groups. The levels of bone Cu were lower than those in control group with significance in low and medium dose groups, [Conclusion]Anti-fluorine agent makes levels of Zn, Ca, Mg increasing in bone, but does not obviously affect levels of Fe, Cu.