UF-1000i尿沉渣分析仪在诊断尿路感染中的应用

Application of UF-1000i Urinary Sediment Analyzer on Diagnosis of Urinary Tract Infection

目的探讨UF-1000i尿沉渣分析仪辅助诊断尿路感染的价值。方法采集908份中段尿液标本,使用UF-1000i尿沉渣分析仪测定尿液白细胞和细菌计数数据,同时进行细菌培养,以细菌培养结果为标准,利用SPSS17.0统计软件绘制受试者工作特征曲线(receive operotiug characteristic curve,ROC曲线),求出白细胞和细菌计数对尿路感染的诊断阈值,并计算其灵敏度、特异性、阳/阴性预测值和假阳性/假阴性率和准确率。结果尿培养结果阳性的标本占39.7%,其中最常见的致病菌是大肠埃希菌。白细胞和细菌计数对尿路感染的诊断阈值分别为91.5/μl和2944.5/μl,白细胞和细菌计数联合测定对尿路感染检查的最佳灵敏度、特异性、假阳性/假阴性率、阳/阴性预测值和准确率分别为63.0%、92.3%、7.7%、37.0%、85.1%、78.2%、80.3%。结论用UF-1000i尿沉渣分析仪,对白细胞和细菌计数联合检测可筛除大部分的阴性结果,减轻工作量,降低成本。

[Objective] To study the value of UF-1000i urinary sediment analyzer on assisted diagnosis of urinary tract infection.[Methods]908 specimens of medistream urine were examined for leukocyte and bacteria counts by UF-1000i urinary sediment analyzer. Meanwhile, bacterial culture was conducted. According to the results of bacterial culture, ROC curve was drawn with SPSS 17.0 software, the threshold values of leukocyte and bacteria counts for diagnosis of urinary tract infection were found out, and its sensitivity, specificity, positive/ negative predictive value, false positive/ false negative rate and accuracy were calculated.[Results]The positive rate of urine culture was 39.7%, and the most common pathogen was Escherichia coli. The threshold value of leukocyte and bacteria counts for diagnosis of urinary tract infection was 91.5/μl and 2944.5/μl respectively. The optimum sensitivity, specificity, false positive/ false negative rate, positive/ negative predictive value and accuracy of the combined determination of leukocyte and bacteria counts for urinary tract infection was 63.0%, 92.3%, 7.7%, 37.0%, 85.15, 78.2% and 80.3%,respectively.[Conclusion]With UF-1000i urinary sediment analyzer, the combined determination of leukocyte and bacteria counts can remove the great mass of negative results, reduces workload and cost.