HPLC测定新疆不同产地唇香草中齐墩果酸和熊果酸的含量

Determination of Oleanolic Acid and Ursolic Acid in Ziziphora Clinopodioides by HPLC from Different Districts in Xinjiang

目的:建立维药唇香草中齐墩果酸、熊果酸的含量测定方法。方法:采用高效液相色谱法,色谱柱ODS C18柱(4.6 mm×150 mm,10μm),流动相甲醇-0.03 mol.L-1磷酸盐缓冲液(90∶10,pH 3),检测波长214 nm,流速0.5 mL·min-1,进样量10μL。结果:齐墩果酸在0.4～1.2 g·L-1线性关系良好,r=0.999 6,平均回收率99.5%,RSD 1.19%;熊果酸在0.6～1.8 g·L-1线性关系良好,r=0.999 6,平均回收率102.3%,RSD 1.25%。结论:本方法简单快捷,适用于测定唇香草中齐墩果酸和熊果酸的含量。

Objective：To establish an HPLC method for the simultaneous determination of oleanolic acid and ursolic acid in Uygur medicine Ziziphora clinopodioides.Method：A ODS C18（4.6 mm × 150 mm,10 μm）column was adopted.The mobile phase consisted of methanol and 0.03 mol·L^-1 phosphate buffer（pH 3,90：10）.Detection wavelength was at 214 nm.The flowrate was 0.5 mL·min-1.The injection volumn was 10 μL.Result：oleanolic acid was linear in the range was 0.4-1.2 g·L^-1（r = 0.999 6）.ursolic acid was linear in the range was 0.6-1.8 g·L^-1（r = 0.999 6）,and the linear relationship is accurate.The average recovery（n = 6） of oleanolic acid was 99.5%（RSD 1.19%） and ursolic acid was 102.3%（RSD 1.25%）.Conclusion： The method was simple,feasible and reproducible,and can be used for the simultaneous determination of oleanolic acid and ursolic acid in Z.clinopodioides.