摘要
目的:建立维药唇香草中齐墩果酸、熊果酸的含量测定方法。方法:采用高效液相色谱法,色谱柱ODS C18柱(4.6 mm×150 mm,10μm),流动相甲醇-0.03 mol.L-1磷酸盐缓冲液(90∶10,pH 3),检测波长214 nm,流速0.5 mL·min-1,进样量10μL。结果:齐墩果酸在0.4~1.2 g·L-1线性关系良好,r=0.999 6,平均回收率99.5%,RSD 1.19%;熊果酸在0.6~1.8 g·L-1线性关系良好,r=0.999 6,平均回收率102.3%,RSD 1.25%。结论:本方法简单快捷,适用于测定唇香草中齐墩果酸和熊果酸的含量。
Objective:To establish an HPLC method for the simultaneous determination of oleanolic acid and ursolic acid in Uygur medicine Ziziphora clinopodioides.Method:A ODS C18(4.6 mm × 150 mm,10 μm)column was adopted.The mobile phase consisted of methanol and 0.03 mol·L^-1 phosphate buffer(pH 3,90:10).Detection wavelength was at 214 nm.The flowrate was 0.5 mL·min-1.The injection volumn was 10 μL.Result:oleanolic acid was linear in the range was 0.4-1.2 g·L^-1(r = 0.999 6).ursolic acid was linear in the range was 0.6-1.8 g·L^-1(r = 0.999 6),and the linear relationship is accurate.The average recovery(n = 6) of oleanolic acid was 99.5%(RSD 1.19%) and ursolic acid was 102.3%(RSD 1.25%).Conclusion: The method was simple,feasible and reproducible,and can be used for the simultaneous determination of oleanolic acid and ursolic acid in Z.clinopodioides.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第16期33-35,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
新疆维吾尔自治区自然科学基金(200821130)
关键词
唇香草
高效液相色谱法
齐墩果酸
熊果酸
含量测定
Ziziphora clinopodioides Lam.; HPLC; oleanolic acid; ursolic acid; determination