HPLC测定柴胡健肝胶囊中黄芩苷的含量

Determination of Baicalin in Chaihu Jiangan Capsules by HPLC

目的:建立HPLC测定柴胡健肝胶囊中黄芩苷含量的方法。方法:色谱柱为Diamonsil C18(4.6 mm×250 mm,5μm),流动相甲醇-水-冰醋酸(54∶46∶0.2),流速1.5 mL·min-1,测定波长315 nm。结果:黄芩苷线性范围为8.072～48.43 mg·L-1,平均回收率为99.54%,RSD 0.88%(n=6)。结论:本法方便、快速、准确,可用于柴胡健肝胶囊的质量控制。

Objective： To establish an HPLC method for determination of baicalin in Chaihu Jiangan Capsules.Method：The chromatogram column was Diamonsil C18（4.6 mm × 250 mm,5 μm）,the mobile phase composition was the methanol-water-glacial acetic acid（54∶46∶0.2） the flow rate was 1.5 mL·min^-1,the detection wavelength was at 315 nm.Result：The linear range of baicalin was 8.072 ～ 48.43 g.L-1,the average recovery was 99.54% with RSD 0.88%（n = 6）.Conclusion：the method was convenient,quick and accurate.It can be used for quality control of Chaihu Jiangan Capsules.