复方半边莲生物活性研究

Studies on Biological Activity of Compound Recipe of Lobelia Chinensis

目的:研究复方半边莲对α-葡萄糖苷酶抑制、抗氧化及抗菌活性。方法:采用大孔吸附树脂柱色谱法,以不同体积分数甲醇水溶液洗脱,利用96微孔板法检测α-葡萄糖苷酶抑制活性;采用DPPH、ABTS和FRAP方法评价抗氧化活性;采用K-B法和倍比稀释法,测定其抗菌活性。结果:活性成分主要集中在100%,60%和40%甲醇洗脱部位。100%甲醇洗脱部位F(IC50=411.50 mg·L-1)和G(IC50=543.70 mg·L-1)抑制α-葡萄糖苷酶的活性高于阳性对照Acarbose(IC50=1 081.27 mg·L-1);100%甲醇洗脱部位F清除ABTS自由基的能力(IC50=11.23 mg·L-1)和G(IC50=10.81 mg·L-1)略低于阳性对照BHT(IC50=7.47 mg·L-1);100%甲醇洗脱部位G和60%甲醇洗脱部位E对金黄色葡萄球菌(SA)的抑制能力最强,MIC均为62.5μg.disc-1。结论:首次对复方半边莲进行生物活性研究,发现其有较好的α-葡萄糖苷酶抑制、抗氧化和抗菌活性。

Objective： To investigate the α-glucosidase inhibitory,antioxidant and antibacterial activity of compound recipe of Lobeliae chinese（CRLC）.Method： Fractions were eluted by methnol-water in macroporous resin.All the fractions were assayed for the inhibitory activity of α-glucosidase,antioxidant activity and antibacterial activity by the method of 96-microplates,DPPH,ABTS and FRAP,K-B and dilution method respectively.Result： Active fractions were mainly concentrated in the 100%,60% and 40% methanol-water eluent.100% methanol eluent F（IC50 = 411.50 mg·L^-1 and G（IC50 = 543.70 mg.L-1） were higher than that of Acarbose（IC50 = 1 081.27 mg·L^-1 which was used as positive control on inhibitory activity of α-glucosidase.100% methanol eluent F（IC50 = 11.23 mg·L^-1 and G（IC50 = 10.81 mg·L^-1 had good ABTS free radical scavenging activity,which was slightly lower than that of BHT（IC50 = 7.47 mg·L^-1 as positive control.100% methanol eluent G and 60% methanol eluent E had the highest antibacterial of SA,both of MIC were 62.5 μg.disc-1.Conclusion：Bioactivity on CRLC was assayed for the first time,and it had good α-glucosidase inhibitory activity,antioxidant activity and antibacterial activity.