降糖消脂片对糖尿病动物降糖作用的研究

Study of Jiangtang Xiaozhi Tablets on Decreasing of Blood Glucose in Rat and Mouse Models of Diabetes Mellitus

目的:采用糖尿病大、小鼠模型,观察降糖消脂片的降糖作用。方法:大鼠用链脲佐菌素iv造模(50 mg·kg-1)。小鼠用四氧嘧啶iv造模(90 mg.kg-1)。3 d后测血糖,筛选成模动物。大鼠分为7组,12只/组。连续ig降糖消脂片8,4,2 g·kg-1 8周,测定血糖、糖化血红蛋白、胰岛素、胰高血糖素等指标。小鼠分为7组,14只/组。连续ig降糖消脂片10,5,2.5 g·kg-1 4周,测血糖。结果:糖尿病模型大鼠在连续ig 8周后,降糖消脂片各剂量组与模型组比较,血糖明显降低(P<0.05);高剂量组糖化血红蛋白、胰高血糖素明显下降(P<0.05),胰岛素水平有增高的趋势;胰腺病理结果显示:降糖消脂片高剂量组胰岛数目明显增多。糖尿病小鼠模型在连续ig 4周后,降糖消脂片高、中剂量组血糖明显降低(P<0.05～0.001)。结论:降糖消脂片对糖尿病大、小鼠均有明显降糖作用。

Objective：To observe effects of Jiangtang xiaozhi Tablets on decreasing levels of blood glucose in rat and mouse models of diabetes mellitus.Method：Rats were intravenously given streptozotocin at dose of 50 mg·kg^-1.Mice were intravenously given Alloxan at dose of 90 mg·kg^-1to induce hyperglycemia.The levels of blood glucose in the rats and mice were measured after 3 days for screening model animals.Thereafter,rats were divided into 7 groups（n = 12,each） and were continuously given Jiangtang xiaozhi tablets by intragastric administration for 8 weeks.The levels of blood glucose,HBAlc,insulin and glucagon were assayed.While mice were divided into 7 groups（n=14,each） and were continuously given Jiangtang xiaozhi tablets by intragastric administration for 4 weeks.The level of blood glucose was assayed.Result： The levels of blood glucose in each Jiangtang xiaozhi treatment group,HBAlc and glucagon levels in high dose group were significantly decreased（P〈0.05） comparing to those of model rats while serum insulin levels were increased in the high dose group.The morphological study of pancreas in rats demonstrated that number of islets markedly increased in the high dose group.The levels of blood glucose in mice were significantly decreased（P〈0.05-0.001） in middle and high dose groups comparing to that of model mice.Conclusion：Jiangtang xiaozhi Tablets has obvious effect on decreasing blood glucose for model animals of diabetes mellites.