抑郁症睡眠障碍模型大鼠甲状腺激素变化及逍遥散干预的研究

Expression of Serum Thyroxine in a Rat Model of Sleep Deprivation Depression and Influenced of Ease Powder Decoction

目的:观察抑郁症睡眠障碍(SDD)大鼠自主行为及血清TT3(三碘甲状腺原氨酸),TT4(甲状腺素),FT3(游离三碘甲状腺原氨酸),FT4(游离甲状腺素),TSH(促甲状腺素)表达变化,探讨甲状腺激素水平与抑郁症睡眠障碍的关联及逍遥散的干预作用。方法:48只Wistar大鼠进行敞箱行为(OFT)试验,根据得分将大鼠随机分为4组,正常组、模型组、米氮平组、逍遥散组。除正常组外,其余各组共接受21 d不同的应激。造模开始当天应激前1 h模型组、米氮平组、逍遥散组分别给予等容量生理盐水、米氮平、逍遥散水提液。21 d计算行为得分后处死大鼠,取大鼠血清置-20℃冰箱中备测TT3,TT4,FT3,FT4,TSH水平。结果:21 d后模型组自主活动较应激前显著减少(P<0.01),与模型组比较,逍遥散组及米氮平组大鼠的自主活动明显增加(P<0.01),2组与正常组无差异;各组血清TT3,TT4水平变化无差异;模型组FT3水平下降,FT4水平上升,TSH水平无变化,米氮平组、逍遥散组与模型组比较,无统计学意义。结论:逍遥散具有缓解抑郁症睡眠障碍的效应,但对甲状腺激素水平变化无影响。

Objective：To discuss the molecular mechanism of Ease Powder decoction treating on sleep deprivation depression by observing the changes of autonomic-behavior and the expression of serum TT3,TT4,FT3,FT4,TSH in a rat experimental model of sleep deprivation depression（SDD）.Method：Fouty eight Wistar rats were divided into four groups after first open-field testing（OFT）,they were normal group,model group,mirtazapine group and Ease Powder decoction group.Except the normal group,the other groups were received different stress for 21 days.Making day stress before 1 h the model group,mirtazapine,Ease Powder decoction group were intragastric administration of normal saline,mirtazapine,Ease Powder decoction respectively.At the end of 21 days,all the animals were sacrificed.The serum of rats was taken then kept in-20 ℃ freezer and the levels of TT3,TT4,FT3,FT4 and TSH were determined.Result：Compared with the situation before SDD,there was a significant decrease in the autonomic-behavior（P 〈 0.01） of SDD after 21 days.Compared with the model group,the autonomic-behavior of Ease Powder decoction group and the mirtazapine group was increased（P 〈 0.01）.Compared with the normal group,there was no difference.The expression of serum TT3 and TT4 was no differences between the groups.However,compared with that in the nomal group,the expression of FT3 in the model group was decreased,FT4 wasincreased,TSH was nochange.Compare with the model group,the mirtazapine group and Ease Powder decoction group had no statistically significant,while the nomal group has statistically significant（P 〈 0.05）.Conclusion：The Ease Powder decoction exert an effect on SDD.However,The Ease Powder decoction has no influence on the expression of thyroxine.