佛甲草对实验性肝纤维化大鼠脂质过氧化的影响

Effect of Sedum lineare on Lipid Peroxidation in Rats with Liver Fibrosis

目的:探讨佛甲草提取液(SLT)对实验性肝纤维化大鼠脂质过氧化的影响。方法:雄性SD大鼠随机分为正常对照组、模型组、阳性对照组(Col,0.1 mg·kg-1)、SLT低剂量组(SLT,4 g·kg-1)、SLT高剂量组(SLT,8 g·kg-1)。采用50%的CCl4花生油溶液灌服制备大鼠肝纤维化模型,期间给予灌服SLT(4,8 g·kg-1)、Col(0.1 mg·kg-1)进行干预,共9周。9周后处死大鼠取固定部位肝组织及血清标本,光镜下观察肝细胞结构和肝纤维化程度,分光光度法检测肝组织匀浆、血清中丙二醛(MDA)含量以及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性的变化。结果:光镜下可见模型组大鼠肝细胞严重变性、坏死,胶原纤维明显增加;肝组织、血清中MDA含量增高,SOD、GSH-Px活力显著下降。SLT预防给药明显改善大鼠肝细胞结构,减轻肝纤维化程度;明显降低肝纤维化大鼠肝脏及血清MDA含量,显著升高SOD、GSH-Px活性。结论:佛甲草提取液对大鼠肝纤维化有一定的防治作用,其机制可能与抗脂质过氧化损伤有关。

Objective： To investigate the effect of extract from Sedum lineare Thunb（SLT） on lipid peroxidation in rat with hepatic fibrosis.Method： Male SD rats were divided into six groups randomly.The hepatic fibrosis model was induced by intragastric administration with 50% carbon tetrachloride dissolved in peanut oil.Once the model was formed,the rat was administrated with various doses of SLT（4 g·kg^-1,8 g·kg^-1） or NS through intragastric pathway,and the process was lasted for 9 weeks.After the last administration,the rat＇s blood and liver are taken under anesthesia.The cellular structure and grade of hepatic fibrosis were observed by histological method,as well as the content and activity of MDA,SOD,GSH-Px in serum and hepatic tissue were detected.Result： In the model group,severe degeneration and necrosis of hepatic cells,significant accumulation of collagen fibers was observed,the level of MDA in hepatic tissue and serum was increased,and the activity of SOD and GSH-Px was decreased obviously.After treated with SLT,degeneration and necrosis of hepatic cells and the grade of hepatic fibrosis were inhibited,the level of MDA was descended,and the activity of SOD and GSH-Px was enhanced significantly.Conclusion：SLT has a therapeutic effect on hepatic fibrosis in rats,the mechanism is possibly related with alleviation of the injury induced by lipid peroxidation.