不同采收期大花罗布麻叶中总黄酮的含量测定

Determination of Flavonoid of Poacyzuan hendersonii Leaves from Different Harvest Time

目的:比较不同采收期大花罗布麻叶中总黄酮的含量并评价出最佳采收期。方法:采用超声法提取大花罗布麻叶中的总黄酮,紫外-可见分光光度法于508 nm下测定。结果:芦丁在22.78～113.88 mg.L-1内呈良好的线性关系(r=0.999 8),平均加样回收率为98.87%,RSD为1.03%(n=9);大花罗布麻叶不同采收期的测定结果为:4～5月份相对较低,6～9月份稳步增长,10月份相对9月份有所降低。结论:本方法简便、准确,重现性好,可用于评价大花罗布麻叶的最佳采收期,为该药材合理开发利用提供科学依据。

Objective： Comparing the content of flavonoid in different harvesting periods and to evaluate the optimal harvest time.Method：Extraction of flavonoid with ultrasonic.The content of flavonoid was compared by UV at wavelength of 508 nm.Result：Rutin was linear in the range of 22.78-113.88 mg.L-1（r = 0.999 8） with average recovery of 98.87% and RSD of 1.03%（n = 9）.The measurement results of Poacyzuan hendersonii leaves in different harvesting time：relative low in April and May,unfaltering rose in June to September,little lower in October than in September.Conclusion：The method was simple,accurate,reproducible,it can be used to evaluate the best harvest time of P.hendersonii,and provide a scientific basis for rational development and utilization.