3种前体饲喂对高山红景天悬浮培养细胞中红景天苷的影响

Effects of Three Kinds of Precursors on Cell Growth and Salidroside Content of Rhodiola sachalinensis in Cell Suspension Culture

目的:研究前体饲喂对高山红景天细胞生长和红景天苷含量的影响。方法:在细胞培养起始期分别添加苯丙氨酸、肉桂酸和酪氨酸3种前体,在细胞生长的稳定期第14 d收获细胞,测定细胞干重、红景天苷含量、多酚氧化酶(PPO)和苯丙氨酸裂解酶(PAL)的活性。结果:苯丙氨酸的最佳添加质量浓度为20 mg·L-1,肉桂酸和酪氨酸的最佳添加质量浓度均为10 mg·L-1,此时红景天苷含量分别为2.65,3.50,3.21 mg·g-1。但高浓度的酪氨酸对细胞的生长有一定的抑制作用。结论:3种前体在合适的浓度下均能促进细胞中红景天苷的生物合成,并提高多酚氧化酶(PPO)、苯丙氨酸裂解酶(PAL)的活性,其中肉桂酸作用明显。

Objective：To study the effect of precursor feeding on cell growth and salidroside content of Rhodiola sachalinensis A.Bor.Method：Three kinds of precursor of L-phenylalanine,cinnamic acid and tyrosine were added in the initial period of cell culture and cells were harvested to measure dry weight,salidroside content,polyphenol oxidase（PPO） and phenylalanine lyase（PAL） activity in the 14 d,i e in the stability phase of the cell growth curve.Result：The best concentrations of phenylalanine,cinnamic acid and tyrosine to be added were 20,10,10 mg·L-1,while salidroside content was 2.65,3.50,3.21 mg·g-1 respectively.But high concentration of tyrosine would restrain cell growth.Conclusion：Three kinds of precursor in appropriate concentration could all promote the biosynthesis of salidroside and increase polyphenol oxidase（PPO）,phenylalanine lyase（PAL） activity,among them,cinnamic acid did work obviously.