安子合剂对环磷酰胺诱导小鼠血清抗心磷脂抗体阳性的治疗作用

Therapeutic Effect of Anzi Mixture on Positive Serum Anticardiolipid Antibody in Cyclophosphamide-treated Mice

目的:观察安子合剂对环磷酰胺诱导模型小鼠血清抗心磷脂抗体(ACA)含量的影响。方法:以环磷酰胺作为诱导剂建立小鼠血清ACA阳性模型;空白组给予生理盐水(0.01 mL·g-1)并蒸馏水(0.01 mL·g-1);模型组给与环磷酰胺(0.1mL/10 g)并蒸馏水(0.1 mL/10 g);各治疗组均在环磷酰胺模型基础上分别给与予阿司匹林和高中低三种不同剂量安子合剂(0.1 mL/10 g)。所有组别均每日给药1次,连续14 d。各组小鼠均于第14 th给药后1 h摘眼球取血,离心后分离出血清,ELISA检测法测定血清ACA含量。结果:与空白组比较,模型组小鼠血清ACA含量显著升高(P<0.05);与模型组比较,安子合剂高中低各剂量组小鼠血清ACA含量均显著降低(P<0.05);与阿司匹林组比较,安子合剂各剂量组ACA含量无显著差异。结论:本实验成功建立了环磷酰胺诱导小鼠血清ACA阳性模型;安子合剂能有效降低模型小鼠血清ACA含量,并呈一定量效关系;安子合剂作用强度与阿司匹林相当。

Objective：To investig,ate the therapeutic effect of Anzi Mixture on concentration of positive serum anticardiolipid antibody（ACA） in cyclophosphamide-treated mice.Method：Cyclophosphamide was given to induce positive serum ACA for the model mice.Control group was given saline（0.01 mL.g-1） and distilled water（0.01 mL·g-1）;model group was received cyclophosphamide（0.01 mL·g-1）and distilled water（0.01 mL·g-1）.The treatment groups were given aspirin or high,middle,low doses of Anzi Mixture（0.01 mL·g-1） on the model of cyclophosphamide induced.All groups were administered once daily for 14 consecutive days.Eyeball blood sample was collected for all the mice in all groups 1 h after administration on the 14 th day,and then serum was separated by centrifugation,ELISA was employed to detect the serum ACA levels.Result：Compared with the model group,sig-nificant decrease in serum ACA level was observed in all groups of different dosages of Anzii Mixture（P 〈 0.05）.Compared to aspirin group,there was no obvious difference in serum ACA level for any group of Anzi Mixture.Con-clusion：Anzi Mixture could effectively reduce serum ACA level in cyclophosphamide-treated mice with a dose-de-pendent manner.The potency of Anzi Mixture treatment appeared to be equal to that of aspirin treatment.