丹芪颗粒干预干燥综合征模型大鼠颌下腺水通道蛋白-5的表达研究

Intervention of Danqi Granule on Expression of Aquaporin-5 in Submandibular Glands of Rat Model with Sjogren’s Syndrome

目的:以干燥综合征(SS)模型大鼠颌下腺水通道蛋白-5(AQP5)的表达为靶点,探讨丹芪颗粒对SS的作用机制。方法:自身同种鼠抗原合并百白破疫苗加强免疫法免疫模型动物;离体颌下腺灌流法测试中药丹参、黄芪对唾液腺刺激程度;用Western blot分析SS大鼠颌下腺AQP5的表达。结果:免疫动物颌下腺出现类似SS的病理改变,存在大量淋巴细胞浸润,提示免疫造模成功。颌下腺中药灌流提示丹参、黄芪有不同程度促进唾液腺分泌的作用。Western blot检测显示实验各组均出现相对分子质量为42×103的条带。空白对照组AQP5表达数值正常1.35±0.13,与空白对照组比较,造模生理盐水组AQP5表达数值1.03±0.08明显减少(P<0.05)。造模丹芪高、低剂量组AQP5表达数值较造模生理盐水组明显增强1.31±0.15,1.27±0.10,P<0.05。结论:离体颌下腺中药灌流提示丹参有轻度对唾液腺直接促进作用,黄芪也有类似的作用,但两者促进唾液分泌的图形是不同的。丹芪颗粒可能通过增强颌下腺水分子通道的释放,上调AQP5的表达,从而改善唾液腺分泌功能、增加唾液流量,缓解干燥综合征口腔干燥的症状。

Objective：To investiga：te the mechanism of Danqi Granule for treatment of Sjogren＇s syndrome（SS） in rat,and to consider expression of aquaporin-5（AQP5） as the target point.Method：Submandibular gland tissue was extracted from Wistar rat before blending and emulsification with complete Freund＇s adjuvant（CFA）.After that,subcutaneous injection of DTP vaccine was conducted on the back to enhance immunity.The effects of Astragalus and Danshen on rat isolated salivary glands were investigated,the method used was Chinese herb perfusion.The expression distribution of AQP5 in submandibular salivary glands of rat with SS was shown by Western blot analysis.Result：After immunization 7 weeks of SS rats,thus pathological changes similar to the salivary gland in human SS were found.It suggested that a large number of lymphocyte cells were different invasive depth and effective immunization.Through Chinese herb perfusion on rat isolated salivary glands,it was found that Astragalus and Danshen could promote salivary gland secretion in different depth.Western blot analysis showed that there was a 42 × 103 in each groups.There was no abnormal expression of AQP5 in the control group,Compared with the control group,the expression of AQP5 in the rat model group was significant reduced（P 〈 0.05）;Danqi Granule of high-dose and low-dose could significantly increase the expression of AQP5（P 〈 0.05）.Conclusion：The results indicate Danshen can promote salivary glands slightly.Astragalus also has the similar effect.However,they promote the secretion of saliva graphics in different patterns.Danqi Granule through enhanced the release of water molecules in channel of submandibular gland,by upregulating the expression of AQP5,improving the secretive function of salivary glands and increasing saliva flow,so as to release the thirsty symptoms of SS.