补肾温阳化瘀方对子宫内膜异位症模型大鼠内膜组织缺氧诱导因子-1α、葡萄糖转运因子-1表达的影响

Effect of Bushen Wenyang Huayu on Expression of HIF-1α and GLUT-1 in Rat Model of Endometriosis

目的:探讨补肾温阳化瘀方治疗内异症的可能作用机制。方法:选用SPF级雌性大鼠复制腹膜型内异症大鼠模型,将造模成功的大鼠分为4组:补肾温阳化瘀方高剂量组(16只)、低剂量组(16只)、丹那唑组(16只)、模型组(17只),并设对照组(12只)。运用免疫组化(S-P)法,检测内异症模型大鼠治疗前、后异位内膜组织中缺氧诱导因子-1α(HIF-1α)、葡萄糖转运因子-1(GLUT-1)的表达,并比较治疗前后异位灶体积变化。结果:补肾温阳化瘀方高、低剂量组及丹那唑组治疗后子宫内膜异位灶体积较治疗前明显缩小(P<0.05),且以补肾温阳化瘀方高剂量组最显著(P<0.01);HIF-1α,GLUT-1在模型组异位内膜的表达与对照组在位内膜相比显著升高(P<0.01);补肾温阳化瘀方高、低剂量组及丹那唑组均能够显著抑制HIF-1α,GLUT-1的高表达(P<0.01),与对照组表达无明显差异,且以中药高剂量组作用效果最显著,低剂量组次之;丹那唑组与补肾温阳化瘀方高剂量组比较抑制效果有差异(P<0.05)。结论:补肾温阳化瘀方可能通过抑制HIF-1α与GLUT-1的表达达到治疗内异症的目的。

Objective：To explore the mechanism of Bushen Wenyang Huayu（BSWYHY） on endometriosis（EMs）.Method： The peritoneal type endometriosis in SPF-grade female rats was established.They were then divided into 4 groups： BSWY high dose group（16）,BSWYHY low dose group（16）,danazol group（16）,model group（17）,and control group（12）.Immunohistochemistry was performed to examine the expression of HIF-1α and GLUT-1 in the ectopic endometrial tissues of the EMs model rats before and after treatment,and the volume change in endometriotic lesions before and after treatment was compared.Result：After treatment with high dose and low dose of BSWY and danazol,volumes of ectopic lesions were reduced obviously（P 〈 0.05）,and the volume of the high dose group demonstrated the most notable reduction（P 〈 0.01）.The expression of HIF-1α and GLUT-1 in ectopic EMs of the model group is significantly higher than that in eutopic endometrium in the control group（P 〈 0.01）.The BSWYHY high and low dose group and the danazol group were able to inhibit the high expression of HIF-1α and GLUT-1 substantially（P 〈 0.01）,with the high dose group as the best,followed by the low dose anddanazol group（P 〈 0.05）.Conclusion： BSWY can be an effective measure for reducing the high expression of HIF-1α and GLUT-1 for the treatment of EMs.