抗核糖体P0蛋白抗体在系统性红斑狼疮诊断中的临床应用

Anti-ribosomal P0 Protein Antibodies in Systemic Lupus Erythematosus and Their Clinical Diagnosis Significance

目的研究抗核糖体P0蛋白抗体对系统性红斑狼疮(SLE)的临床诊断意义。方法分别用基因重组的核糖体P0蛋白和纯化的核糖体P蛋白作为抗原检测126例SLE患者和145例非SLE自身免疫病患者血清中抗P0抗体和抗P抗体;同时对126例SLE患者采用线性免疫分析检测抗dsDNA抗体、抗SmD1抗体、抗核小体抗体、抗SSA60抗体、抗SSB抗体等,比较以上抗体在系统性红斑狼疮临床诊断中的意义。结果 SLE患者抗核糖体P0蛋白抗体的阳性率为38.89%,非SLE自身免疫病患者组为2.07%,两组之间差异有统计学意义(χ2=56.59,P<0.01);SLE患者组中,抗P0阳性率高于抗P蛋白抗体阳性率(χ2=19.36,P<0.001);抗核糖体P0蛋白抗体诊断SLE的特异性为97.93%,抗dsDNA抗体和抗核糖体P0蛋白抗体单独阳性率较高,分别为15.08%和9.52%;抗SmD1抗体和抗核小体抗体单独阳性率较低,分别为3.17%和3.97%。结论抗核糖体P0蛋白抗体对SLE患者有高特异性和较高的敏感性,对抗dsDNA抗体、抗SmD1抗体、抗核小体抗体等抗体阴性的SLE患者有尤其重要的临床应用价值。

OBJECTIVE To study the clinical diagnosis significance of the anti-ribosomal P0 protein antibodies in systemic Lupus Erythematosus （SLE）. METHODS The ribosome protein zero （PO） of gene recombination and purified ribosome P protein were used as the antigens to detect the anti-ribosomal P0 protein antibodies and the anti-ribosomal P protein antibodies in 126 SLE sufferers and 145 non-SLE autoallergic disease sufferers. To detect the anti-dsDNA antibodies, the anti-SmD1 antibodies, the nuclear body antibodies and so on in 126 SLE sufferers by line immunoassay at the same time and to compare the significance of these antibodies in the clinical diagnosis of SLE. RESULTS The positive rate of the anti-ribosomal P0 protein antibodies was 38. 89% in SLE sufferers vs 2.1% in non-SLE autoallergic disease sufferers. The difference of two groups had statistical significance （x^2= 19.36,P〈0. 001）. The specificity of the anti-ribosomal P0 protein antibodies to diagnose SLE was 97.93 %. The positive rate of the anti-ribosomal P0 protein antibodies, the anti-dsDNA antibodies, the anti-StuD1 antibodies and the nuclear body antibodies was 9.52% and 15.08% and 3. 17% and 3.97%, respectively. CONCLUSIONS The anti-ribosomal P0 protein antibodies have significant clinical value in diagnosis of SLE.