米非司酮对人胎盘绒毛滋养层细胞周期动力学的影响

Effect of Mifepristone on Cell-Cycle Kinetics of Human Placental Chorio-trophoblastic Cell

目的对正常早孕吸宫流产和顿服米非司酮１５０ｍｇ４８小时后的吸宫流产物的绒毛组织各２６例进行细胞周期动力学的分析。方法采用流式细胞分析技术进行分析。结果服用米非司酮后，其胎盘绒毛滋养层细胞的ＤＮＡ倍体水平无变化，而Ｇ０、Ｇ１期细胞明显增加（Ｐ＝０００７），Ｇ２、Ｍ期明显降低（Ｐ＝００４２），Ｓ期亦有下降趋势，但统计学处理无显著性意义（Ｐ＝００５４）。结论米非司酮对人胎盘绒毛滋养层细胞周期动力学有直接的作用，通过阻断滋养层Ｇ０、Ｇ１期细胞向Ｓ期的转化，从而抑制滋养层细胞的增殖，达到抗早孕的目的。

Objective\ To study the cell cycle kinetics of the early pregnant chorionic villi.Methods\ Flow-cytometry was introduced in our study.26 samples were obtained only by surgical aspiration,and 26 samples were obtained by administration of mifepristone 150mg once per 48 hours before the patient accepted the operation.Results\ We found that the level of the ploidy of DNA in human placental choriotrophoblastic cell had no change between the two groups.The percentage of the cells in G 0+G 1 phase was much higher in the administration group( P =0 007),whereas it was much lower in G 2+M phase( P =0 042).Conclusions\ Mifepristone may block the transformation of the cells from G 0,G 1 phase to S stage in early pregnancy chorionic villi,thus leading to the block of cell cycle.Abortion happens.