林可霉素生物合成基因lmbH的回转化

Backtransformation of lincomycin biosynthesis gene lmbH

以ｐＩＪ７０２或ｐＩＪ４８６为载体构建了３个含有ｌｍｂＨ基因的重组质粒ｐＥＳＳ７０１、ｐＥＳＳ７０４和ｐＥＳＳ７０８，分别回转化林可霉素产生菌（Ｓ．ｌｉｎｃｏｌｎｅｎｓｉｓ）Ｂ４８，并随机挑选了６００个回转化克隆进行摇瓶初筛，以抑菌圈直径作为衡量林可霉素产量的标准。结果发现，每一种重组方案均得到了一个产量明显高于产生菌对照的回转化同源整合菌株，即６号（ｐＥＳＥ７０１）、８１号（ｐＥＳＥ７０４）和２４４号（ｐＥＳＥ７０８），折算成效价的提高率分别为７１．９％，１１０．６％和５３．６％。

Using pIJ702 or pIJ486 as vector, three recombinant plasmids pESS701, pESS704 and pESS708 which all contained lmbH gene were constructed, then they were backtransformed into S.lincolnensis B48 and 600 transformants were screened, respectively. Using the antibacterial inhibition zone to evaluate the lincomycin productivity level, the data indicated that at least one high producing strain was obtained from each backtransformation of the three recombinant plasmids and named as No.6, No.81 and No.244, respectively, when compared with the industrial parent strain. The relevant increasing rates of the lincomycin productivity level were 71.9%, 110% and 53.6%, respectively.