Staurosporine对人低分化鼻咽癌细胞系细胞周期和DNA倍体含量影响的动态观察

Investigation on changes of cell cycle and DNA diploid in CNE 2Z cells affected by staurosporine

目的：探讨蛋白激酶Ｃ（ＰＫＣ）抑制剂Ｓｔａｕｒｏｓｐｏｒｉｎｅ（ＳＴ）诱导ＣＮＥ－２Ｚ细胞凋亡时，对细胞周期的选择性和对ＤＮＡ含量及细胞周期的动态影响。方法：以ＰＫＣ催化区抑制剂ＳＴ作用于ＣＮＥ－２Ｚ细胞不同时间后，收集细胞进行ＰＩ染色和流式细胞仪检测。结果：ＳＴ以２×１０－６ｍｏｌ／Ｌ终浓度分别作用至３，６，１２，２４ｈ后，发现Ｇ２期百分比分别为３０．４３±７．１６、３０．８３±６．２５、４２．４３±３．９８和７１．８０±７．５５，均较对照组１２．０８±３．４９增高明显。比较差异有显著性（Ｐ＜０．０１）；而Ｇ１期在３，６，１２，２４ｈ分别为３４．７０±１．５６，３７．０３±６．４７，１６．６３±１．７７和１５．３０±５．４６，均较对照组６２．５６±６．５７明显降低，比较差异有显著性（Ｐ＜０．０１）。亚二倍体峰百分比在１２、２４ｈ分别为３７．１０±７．２１和４３．７２±６．７３，均较对照组９．８１±１．７８显著增加（Ｐ＜０．０１）；而１２ｈ以前该峰增加不明显。结论：ＳＴ在终浓度为２×１０－６ｍｏｌ／Ｌ诱导ＣＮＥ－２Ｚ细胞至１２ｈ后即可出现明显的ＤＮＡ含量降低的凋亡细胞，对Ｇ２期细胞敏感而使之逐渐阻滞，且存在?

Objective:To investigate the changes of cell cycle and DNA diploid in CNE 2Z cells induced by the inhibitor of protein kinase C(PKC).Methods:CNE 2Z cells were treated by PKC inhibitor staurosporine(ST),acting on the catalytic domain,at the concentration of 2×10 -6 mol/L for various hours,stained with propidium iodide (PI),and tested by flowcytometry (FCM).Results:Within 3,6,12,and 24 hours,the percentages of G 2 phase were 30.43±7.16,30.83±6.25, 42.43 ±3.98 and 71.80±7.55 respectively, all of which were higher than that of control groups 12.08±3.49( P < 0.01). The percentages of G 1 phage were 34.70±1.56,37.03±7.21,16.63±1.77,and 15.30±5.46 respectively, all of which were lower than that of control groups 62.56±6.57( P <0.01).The percentage of hypodiploid was 37.10±7.21 and 43.72±6.73 for 12 and 24 hours respectively,and both obviously increased compared with that of control groups 9.81 ±1.78( P <0.01).Conclusion:The results showed that the number of apoptotic cell induced by ST at the concentration of 2×10 -6 mol/L gradually increased while the G 2 phase increasing and G 1 phase decreasing within 24 hours,and it suggested that ST could induce CNE 2Z cells apoptosis and selectively affect G 2 phase of cell cycle.