用高效液相色谱法测定大鼠血浆中甘草次酸浓度

Determination of the Concentration of Glycyrrhetic Acid in Rat Plasma by HPLC

采用高效液相色谱法测定大鼠血浆中甘草次酸浓度．线性范围０３６～４８ｍｇ／Ｌ．精密度（ＲＳＤ）日内小于１５％，日间小于１６％，回收率９２２％，最低检测浓度为０３ｍｇ／Ｌ．

The contents of glycyrrhetic acid in plasma were determined by HPLC under following conditions:column ODS 5 μm,200 mm×4 6 mm;mobil phase methyl alcohol and 5 mmol/L tetrabutyl ammonium bromide aqueous solution( V∶V =90∶10,pH 3 6);flow rate 1 0 mL/min; λ=248 nm .The result showed the minimum determination concentration of glycyrrhetic acid was 0 3 mg/L.