远志皂苷H对离体平滑肌与心脏的作用

Effects of saponin H isolated form Polygala tenuifolia Willd on isolated smooth muscle and heart

目的：研究一种新的远志皂苷Ｈ对平滑肌和心肌的药理作用及其机制。方法：制备离体兔回肠，胸主动脉条，Ｌａｎｇｅｎｄｏｒｆ心脏，豚鼠气管条和动情期未孕大鼠子宫标本，记录给药３ｍｉｎ后的张力变化。结果：Ｈ对上述４种平滑肌均具兴奋作用，１９和７６μｍｏｌ·Ｌ－１分别使其张力提高６４％和１６７％，３０％和１１８％，２０％和５０％，以及１００％和１８０％；对心脏则呈依剂量的抑制作用，１９，３８和７６μｍｏｌ·Ｌ－１分别使心肌收缩力降低２０％，２３％和３４％，使心率减少１７％，２０％和３５％，但不影响冠脉流量。Ｈ对子宫的兴奋作用不受苯海拉明影响，但可被吲哚美辛部分抑制。结论：Ｈ对平滑肌具兴奋作用，对心肌具抑制作用，其中对子宫的作用可能部分地与前列腺素合成酶有关。

OBJECTIVE:To study the pharmacological effects of saponin H,a new saponin isolated from Polygala tenuifolia Willd,on isolated smooth muscle and herat.METHODS:Isolated rabbit ileum,aorta strip,Langendorff heart,guinea pig trachea,and rat uterus were prepared.Their changes in tension 3 min after administration of H were recorded.RESULTS:19 and 76 μmol·L -1 of H showed contracting on above mentioned smooth muscles,with increasing their tensions by 64% and 167%,30% and 118%,20% and 50%,100% and 118%,respectively.19,38 and 76μmol·L -1 of H showed dose dependent inhibition on heart,decreased contractile force by 20%,23% and 34%,and reduced the heart rate by 17%,20% and 35%,respectively,but failed to affect the coronary flow.The contracting action of H on uterus was not influnced by diphenhydramine,but partly attenuated by indomethacin.CONCLUSION:H excited the isolated smooth muscles but inhibited the heart.Its action on uterus was partly related to the activation of prostaglandin synthetase.