儿童恶性胶质瘤VEGF表达与血管生成及细胞增殖的关系

The Relatuionship between Vascular Endothelial Growth Factor Expressionand Angiogenesis and Cell Proliferationin Children with Malignant Glioma

目的：研究血管内皮细胞生长因子（ＶＥＧＦ）在儿童恶性胶质瘤的表达及其与血管生成，细胞增殖的关系。方法：应用免疫组化方法检测３３例儿童恶性胶质瘤组织中ＶＥＧＦ表达与微血管数（ＭＶＱ）、ＰＣＮＡ标记指数（ＰＣＮＡＬＩ）。结果：３３例儿童恶性胶质瘤ＶＥＧＦ表达阳性２３例（６９．７％）；ＶＥＧＦ表达阳性肿瘤ＭＶＱ、ＰＣＮＡＬＩ显著高于ＶＥＧＦ表达阴性肿瘤（Ｐ＜０．００５）；肿瘤组织ＶＥＧＦ表达与ＭＶＱ、ＰＣＮＡＬＩ显著相关（相关系数ｒ分别为０．５２和０．３７，Ｐ＜０．００１）。结论：儿童恶性胶质瘤细胞可以合成ＶＥＧＦ，ＶＥＧＦ在肿瘤血管生成及细胞增殖中起重要作用。

Objective: To investigate the relation of vascular endothelial growth factor (VEGF) ex-pression with angiogenesis and cell proliferation in childhood malignant glioma. Methods:Immunohisto-chemical technique was used to detect the expression of VEGF, microvessel quantity (MVQ) and PCNAlabelling index (PCNA LI) in 33 childhood malignant gliomas. Results: Positive staining for VEGF wasobtained in 23 out of 33 cases (69- 7% ) . The MVQ and PCNA LI in VEGF - positive tumors were signifi-cantly higher than those in VEGF - negative tumors ( P < 0 . 005) . The expression of VEGF in tumortissues was significantly related with MVQ and PCNA LI ( r - 0. 52 and 0. 37 respectively (P < 0. 001 ) .Conclusion: VEGF can be synthesized in tumor cells of malignant glioma of children and it might play animportant role in angiogenesis and cell proliferation.