用HPLC法测定牛黄上清片中各大黄素成分的含量

Determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Niuhuang Shangqing Tablets by HPLC

目的:建立HPLC同时测定牛黄上清片中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚含量的方法。方法:色谱柱为Shimadzu VP-ODS柱(4.6mm×250mm,5mm),流动相为乙腈-0.1%磷酸梯度洗脱,流速为1.0ml/min,检测波长为254nm,柱温30℃。结果:芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚分别在0.042～0.840μg(r=0.9996)、0.168～3.352μg(r=0.9998)、0.084～1.680μg(r=0.9997)、0.093～1.852μg(r=0.9999)和0.174～3.488μg(r=0.9994)内呈良好线性关系,平均回收率分别为99.8%(RSD=1.34%)、99.8%(RSD=1.72%)、100.0%(RSD=0.89%)、99.5%(RSD=0.97%)和100.2%(RSD=1.04%)。结论:本方法简便、快速、准确,可用于牛黄上清片的质量控制。

Objective：To develop a HPLC method for simultaneous determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Niuhuang Shangqing Tablets.Methods：The sample was separated on the Shimadzu VP-ODS column（4.6 mm×250 mm,5.0 μm） with acetonitrile-0.1% phosphoric acid as the mobile phase in gradient elution.The flow rate was 1.0 mL·min-1,the detection wavelength was 254 nm and the column temperature was 30 ℃.Results：Aloe-emodin,rhein,emodin,chrysophanol and physcion had good linear relationship in the range of（0.042-0.840）μg（r=0.9996）,（0.168-3.352）μg（r=0.9998）,（0.084-1.680）μg（r=0.9997）,（0.093-1.852）μg（r=0.9999） and（0.174-3.488）μg（r=0.9994） respectively.Their average recovery rates were 99.8%（RSD=1.34%,n=5）,99.8%（RSD= 1.72%,n=5）,100.0%（RSD=0.89%,n=5）,99.5%（RSD=0.97%,n=5）,and 100.2%（RSD=1.04%,n=5）,respectively.Conclusion：The method is rapid,simple and accurate,which is applicable for the quality control of Niuhuang Shangqing Tablets.