异氟醚对大鼠海马蛋白激酶A和蛋白激酶C水平的影响

Effect of isoflurane on levels of PKA and PKC in hippocampus in rats

目的评价异氟醚对大鼠海马蛋白激酶A（PKA）和蛋白激酶C（PKC）水平的影响。方法健康雄性sD大鼠36只，月龄3月，体重180～220g，随机分为3组（n=12），I组不给予任何处理，直接进行认知功能测试；Ⅱ组吸入1．2％异氟醚4h，2d后进行认知功能测试；III组吸入1．2％异氟醚4h，2周后进行认知功能测试。采用Morris水迷宫进行大鼠认知功能测试，记录逃避潜伏期。认知功能测试完毕后，处死大鼠，取海马，测定PKA和PKC的表达和活性。结果与I组比较，Ⅱ组逃避潜伏期差异无统计学意义（P〉0．05），Ⅲ组逃避潜伏期延长，Ⅱ组和Ⅲ组海马PKA和PKC的表达下调，活性降低（P〈0．05）；与Ⅱ组比较，Ⅲ组逃避潜伏期延长（P〈0．05），海马PKA和PKC的表达和活性差异无统计学意义（P〉0．05）。结论吸入1．2％异氟醚4h可抑制海马PKA和PKC的水平，从而导致大鼠认知功能障碍。

Objective To investigate the effect of isofluraue on the levels of protein kinase A （PKA） and protein kinase C （PKC） in hippocampus in rats. Methods Thirty-six 3-month-old male SD rats weighing 180- 220 g were randomly divided into 3 groups （ n = 12 each） ： group I underwent the cognitive function test without being pretreated with isoflurane inhalation （group C）; group II and III inhaled 1.2% isofluranc for 4 h and underwent the cognitive function test 2 days and 2 weeks later respectively （group Iso1 , Iso2 ） . Morris water maze was used to assess the cognitive function and the escape latency was recorded. The animals were killed immediately after the test. The hippoeampus was isolated for determination of the expression and activities of PICA and PKC. Results The escape latency was significantly longer in group III than in group I . The expression of PKA and PKC was significantly down-regulated and the activities of PKA and PKC were significantly decreased in group II and III as compared with group I . There was no significant difference in the expression and activities of PKA and PKC between group II and III Conclusion Four hour 1.2 % isoflurane inhalation can decrease cognitive function by inhibiting the levels of PKA and PKC in hippocampus.