人参皂甙Rg1对TGF-β1诱导的肾小管上皮细胞增殖及细胞外基质的影响

Effect of panaxoside Rg1 on rat renal tubular epithelial cell proliferation and ECM production induced by TGF-β1 in vitro

目的:观察人参皂甙Rg1对转化生长因子-β1诱导的肾小管上皮细胞增殖及细胞外基质的影响。方法:MTT法测细胞增殖情况。ELISA法测定培养细胞上清液中Col-Ⅰ,Col-Ⅲ和FN的含量。结果:在10～40ng/mL浓度范围内时间、剂量依赖性的促进肾小管上皮细胞增殖,减少转化生长因子-β1诱导的肾小管上皮细胞对Col-Ⅰ、Col-Ⅲ、FN的分泌。结论:G-Rg1能够促进TGF-β1诱导下的肾小管上皮细胞的增殖,可能与抑制ECM的分泌有关。

Objective：To investigate the effect of panaxoside Rg1 on proliferation and extracellular matrix（ECM） production of rat renal tubular epithelial cells induced by TGF-β1 in vitro.Methods：Rat renal tubular epithelial cells were incubated with different concentrations of panaxoside Rg1 and the same concentration of TGF-β1.Cell proliferation was assessed by MTT colorimetric assay.Fiberonectin（FN） and type Ⅰcollagen and type Ⅲ collagen contents were detected by ELISA.Results：Administration of 10-40mmol/L panaxoside Rg1 into culture medium could significantly promote cell proliferation in time-dependent and dose-dependent manner.Panaxoside Rg1 also decreased the production of FN and Co1Ⅰand Ⅲ.Conclusion：Panaxoside Rg1 could significantly promote renal tubular epithelial cells proliferation,which could be related with suppressing ECM production induced by TGF-β1.