2007～2009年酵母样真菌的分离培养及其耐药性分析

Isolation and identification of yeast-like fungi and their drug resistance analysis during 2007-2009

目的:探讨引起我院儿童感染酵母样真菌的类型及耐药性,指导临床合理用药。方法:对1553份住院和门诊儿童的各种标本分离出的203株酵母样真菌,采用科玛嘉念珠显色培养基培养,用API-20CAUX酵母样真菌鉴定试条进行鉴定,用ATB-FUN-GUS进行药敏试验。结果:酵母样真菌203株中白色假丝酵母菌160株(78.81%),近平滑假丝酵母菌15株(7.39%),新型隐球菌7株(3.45%),光滑假丝酵母菌13株(6.40%),热带假丝酵母菌5株(2.46%),克柔假丝酵母菌1株(0.49%),葡萄牙假丝酵母菌2株(0.99%)。以呼吸内科、血液科、监护室所占比例较大。203株白色假丝酵母菌对5氟胞嘧啶(5-FC)、两性霉素B(AMB)、氟康唑(FCA)、伊曲康唑(ITR)的敏感率分别为100%、100%、94.38%、80%。结论:致儿童真菌感染的念珠菌仍以白色假丝酵母菌为主,临床用药应依据药敏结果合理用药。

Objective：To explore the types of yeast-like fungi causing hospital infection and their drug resistance for guiding the rational drug use in clinic.Methods：1 553 samples from inpatient and outpatient children were cultured and 203 strains of yeast-like gungi were cultured with CHROmagar medium and identified by API-20CAUX strip for yeast-like fungi.The drug sensitive test of Candida was detected with ATB-FUNGUS trip.Results：Of 203 strains of yeast-like fungi,78.81% was Candida albicans（160 strains）,7.39% was Candida parapsilosis（15strains）,3.45% was crytococcus neoformans（11 strains）,6.4% was Candida glabrata（13 strains）,2.46% was Candida tropicalis（5 strains）,0.49% was Candida keusei（1 strains）,0.99% was Candida lusitancae（2 strains）.The most frequently infected wards were the nephrology department,hematology department and intensive care unit.The sensitivity rates of 203 strains of Candida albicans to 5-fluorocvtosinum（5-FC）,amphotericin B（AMB）,fluconazole（FCA） and itracomzole（ITR） were 100%,100%,94.38% and 80% respectively.Conclusion：The pathogen causing children fungal infection in our hospital is mainly Candida albicans,drugs should be rationally selected according to the results of susceptibility test.