摘要
分别用水、乙醇(体积分数分别为25%、50%、75%)和无水乙醇,对苦菜的根、茎、叶、花4个部位进行冷浸提取。测定不同处理条件下的提取率,并用DPPH自由基清除法检测提取物的抗氧化活性;测定提取物的总黄酮和总多酚类物质的含量及其EC50。结果表明:用50%乙醇提取的苦菜各部位提取率最高,不同部位不同溶剂提取物的抗氧化活性均呈明显的剂量关系;提取物的抗氧化活性与其中总黄酮和总多酚的含量有关;不同部位的抗氧化能力大小为:花>叶>茎>根。
Four different parts of Sonchus oleraceus L. （root,stem,leaf and flower） were extracted separately with water and different concentrations of ethanol （25%,50%,75% and 100%） under room temperature. The calculation of extraction efficiency was performed,and the resultant extracts were tested for their DPPH radical scavenging capacity （EC50,half maximal effective concentration） and the contents of total flavonoids. Fifty percent ethanol gave the highest extraction efficiency of ethanol soluble substances from each of these four parts of Sonchus oleraceus L. Different extracts from different parts of Sonchus oleraceus L. all had significantly dosage-dependent antioxidant activity,which was related to the contents of total flavonoids and total polyphenols. The order of antioxidant activity for these four parts was：flower leaf stem root.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第19期45-48,共4页
Food Science
基金
泰安市2007中药现代化科技专项(5012100999)
关键词
苦菜
总黄酮
总多酚
抗氧化活性
Sonchus oleraceus L.
total flavonoids
total polyphenols
antioxidant activity
