虾头自溶产物苦味与蛋白平均疏水度的联系及脱苦

Analysis of Bitterness-Average Protein Hydrophobicity Relationship and Debittering of Autolysis Products of Shrimp Head

为了探明虾头自溶产物中苦味肽的分布以及蛋白疏水性与苦味的联系,采用超滤将虾头自溶产物分成不同组分,分别检测其苦味与平均疏水度,并研究自溶过程中添加复合风味蛋白酶协同水解虾头对苦味的改善作用。结果表明:虾头自溶产物的苦味主要来自分子质量为3～5kD的肽;不同分子质量大小的自溶产物,平均疏水度越小苦味越大;添加复合风味蛋白酶协同水解虾头,与自溶比较,其蛋白回收率提高了3.5%,苦味降低了50%。

In order to find out the distribution of bitter peptides in the autolysis products of shrimp head and the relationship between bitterness and their protein hydrophobicity,ultrafitration was employed to fractionate the autolysis products,and the separated fractions were tested for their bitterness and average protein hydrophobicity,and the bitterness-improving effect of synergetic hydrolysis by flavourzyme added during shrimp head autolysis was also investigated. The bitterness of the autolysis products mainly resulted from the presence of peptides with a molecular weight varying from 3 to 5 kD. The autolysis products with smaller average hydrophobicity presented stronger bitterness. Compared with single autolysis,the presence of flavourzyme resulted in an increase in protein recovery by 3.5% and a decrease in bitterness by 50%.