白术多糖对益生菌的促生长作用及结构分析

Promoting Effect of Polysaccharides Isolated from Rhizoma Atractylodis Macrocephalae on the Growth of Probiotics and Structure Analysis

对白术、枸杞、太子参、五味子、党参、地黄、陈皮、灵芝的筛选结果表明,白术浸提液对婴儿双歧杆菌体外促生长效果最为明显。白术经水提(1:20)、醇沉(1:3)、透析(3500D)、脱蛋白(Saveg法)后经DEAE阴离子交换柱层析分离得到中性白术多糖(AMP)。AMP对两大类益生菌的促生长结果表明:AMP具有良好的促生长效果,其中对婴儿双歧杆菌(Bifidobacterium infantis)、青春双歧杆菌(Bifidobacterium adolesentis)、动物双歧杆菌(Bifidobacterium animalis)、植物乳杆菌(Lactobacillus plantarum)的促生长效果最佳,且AMP的促生长作用与其添加浓度有关。AMP经红外光谱分析具有多糖特征吸收峰;经多角度激光散射仪结合凝胶渗透色谱(GPC-MALLS)法测得其重均分子质量(Mw)为5527g/mol,分子质量分布(Mw/Mn)为1.726;酸水解法及高效阴离子交换色谱-脉冲安培检测(HPAEC-PAD)分析表明:AMP为杂多糖,单糖组成为阿拉伯糖、半乳糖、葡萄糖和甘露糖,其物质的量比为13.8:11.8:70.2:4.1。

Among eight common medicinal and edible Chinese herbs,namely Rhizoma Atractylodis Macrocephalae,Fructus Lycii Chinensis,Radix Pseudostellariae,Fructus Schisandrae Chinensis,Radix Codonopsitis,Radix Rehmanniae,Pericarpium Citri Reticulatae and Glossy Ganoderma）,the water extract from Rhizoma Atractylodis Macrocephalae had the most obvious promoting effect on the in vitro growth of Bifidobacterium infantis. A neutral polysaccharide fraction was obtained from Rhizoma Atractylodis Macrocephalae after water extraction （1：20 raw material/water ratio）,absolute ethanol precipitation （1：3 concentrated crude extract/absolute ethanol）,dialysis （through a membrane with a molecular weight cut-off of 3500 D）,Sevag deproteinization and DEAE anion exchange column chromatographic separation. The polysaccharide fraction had a promoting effect on the growth of Bifidobacterium and Lactobacillus,especially on B. infantis,B. animalis,B. adolescentis and Lactobacillus plantarum in a concentration-dependent fashion. The infrared spectral analysis showed that the fraction had characteristic absorption peaks of polysaccharides,and its weight-average molecular weight （Mw） was measured to be 5527 g/mol using GPC coupled with a multiangle laser light scattering photometer （GPC-MALLS） and its molecular weight distribution （Mw/Mn） was 1.726. The analysis using acidic hydrolysis followed by high-performance anion exchange chromatography with pulsed amperometric detection （HPAEC-PAD） revealed that the polysaccharide was a hetero polysaccharide and its monosaccharide composition consisted of rabinose,galactose,glucose and mannose in a molar ratio of 13.8：11.8：70.2：4.1.