曲霉T3-5-1产单宁酶培养条件优化

Optimization of Culture Conditions for Producing Tannase by Aspergillus sp.T3-5-1

以通过60Co诱变得到的曲霉T3-5-1为实验菌株,通过单因素与正交试验,对其产单宁酶的条件进行研究。结果表明:以β-环糊精为碳源、蛋白胨为氮源、培养基初始pH值为5.5、单宁酸质量分数为3.5%、接菌量为5%、摇瓶培养转速120r/min、250mL三角锥瓶中装液50mL培养基、培养温度30℃的培养条件为曲霉T3-5-1的最佳产单宁酶条件。在此条件下,曲霉T3-5-1产单宁酶比活力达到202.16U/mL,比优化前提高约4倍。

The conditions for producing tannase by a strain of Aspergillus sp.T3-5-1,which was derived from 60Co-induced mutation,were optimized using single factor and orthogonal array design methods. The optimal carbon source,nitrogen source,medium initial pH,tannic acid concentration,inocumlum size,rotational speed of shaking flask,medium volume contained in 250 mL shaking flask and culture temperature were β-cyclodextrin,peptone,5.5,3.5%,5%,120 r/min,50 mL /250 mL and 30 ℃,respectively. The maximum tannase activity was up to 202.16 U/mL under these conditions,approximately 4-fold higher than before optimization.