黑曲霉固态发酵花生壳提取水溶性膳食纤维

Optimization of Extraction of Soluble Dietary Fibre from Peanut Hull by Aspergillus niger Solid-state Fermentation

以花生壳为原料,应用黑曲霉固态发酵制备花生壳水溶性膳食纤维。通过对黑曲霉菌株特性及其培养基优化的研究,确定培养温度、菌龄、接种量、培养时间的最佳水平,响应面辅助法获得花生壳水溶性膳食纤维的最佳提取工艺条件为:以8g花生壳为原料,水1.88mL/g原料,(NH4)2SO41.88g/100mL、KH2PO41.88g/100mL和MgSO45.63g/100mL的优化培养基,培养温度27℃、黑曲霉菌龄2.9d、接种量16mL、培养时间9.1d,黑曲霉发酵液水解花生壳酶解率可以达到11.03%,水溶性膳食纤维中已糖的聚合度为152.71%,综合评分为105.48。

In order to promote the high value-added utilization of peanut hull,a byproduct of peanut processing,the solid-state fermentation of Aspergillus niger was employed to extract soluble dietary fibre （SDF） from the resource. The growth curve of Aspergillus niger in standard PDY medium was plotted for determining the optimal seed age. This was followed by optimizing the medium composition by orthogonal array design and four fermentation conditions including temperature,seed age,inoculum size and fermentation period by response surface methodology. The optimal medium composition was found to be composed of 8 g of peanut hull and 1.88-fold volume of water containing 1.88 g/100 mL （NH4）2SO4,1.88 g/100 mL KH2PO4 and 5.63 g/100 mL MgSO4,and the optimal fermentation period and temperature,seed age and inoculum size were 9.1 days,27 ℃,2.9 days and 16 mL,respectively. The resultant hydrolysis efficiency,SDF polymerization degree and comprehensive score were 11.03%,152.71% and 105.48,respectively.