苦荞麦蛋白质对乳腺癌细胞的增殖抑制作用

Anti-proliferative Effect of Tartary Buckwheat Protein Fraction TBWSP31 on Breast Cancer Cells

采用MTT法、HE染色法、扫描电镜法研究苦荞麦蛋白质(TBWSP31)对人乳腺癌Bcap37细胞的增殖抑制作用。结果表明:TBWSP31对人乳腺癌细胞株Bcap37的生长有明显的抑制作用,并且存在时间效应和剂量效应。48h和72h的IC50值分别为43.37、19.75μg/mL。HE染色发现细胞经样品作用后,细胞变形,变小,细胞核固缩、裂解,细胞膜皱褶、卷曲和出泡,并且有细胞膜包裹的凋亡小体生成。扫描电镜下观察细胞表面超微结构,发现细胞出现典型的凋亡形态学特征,细胞表面微绒毛大量减少,有的甚至消失,细胞体积变小,细胞膜皱缩,表面凸起,形成了大量的小泡,有的成为凋亡小体。

The inhibition effect of a protein fraction TBWSP31 prepared from tartary buckwheat as described in our previous study on the proliferation of Bcap37 cells was investigated by MTT assay,HE staining and scanning electron microscopic （SEM） observation. TBWSP31 exhibited a notable anti-tumor activity in dose-and time-dependent manners and was also sensitive to human mammary cancer cell Bcap37 with IC50 values of 43.37 （48 h）μg/mL and 19.75μg/mL （72 h）,respectively. After treated with TBWSP31 for 48 h,Bcap37 cells revealed typical apoptotic characteristics. The HE-stained cells exhibited visible morphological changes such as nuclear fragmentation,chromatin condensation,plasma-membrane blebbing and produc-tion of apoptotic bodies under a light microscope. The SEM observations revealed that the decrease of villus,even partial disappearance,plasma-membrane blebbing and production of apoptotic bodies.