α-酪蛋白水解产物对高血压大鼠血压的影响

Effect of α-Casein Hydrolysate on Blood Pressure in Spontaneously Hypertensive Rats

研究水解α-酪蛋白所得水解产物对原发性高血压大鼠(SHR)血压的影响。用α-酪蛋白水解产物以低、中、高3个剂量(0.0245、0.1225、0.6125g/kgbw)分别灌胃SHR大鼠,0.6125g/kgbw灌胃正常Wistar大鼠,测定灌胃后1～8h尾动脉收缩压(SBP)。结果显示,单次灌胃8h内,高剂量α-酪蛋白水解产物对正常Wistar大鼠的血压无影响;3种剂量对SHR的血压升高有明显抑制作用,且高剂量组在0～6h内降压效果最佳,SBP维持在较低水平的时间最长,达到6h,SBP值下降了(29.50±1.0)mmHg。连续灌胃高剂量α-酪蛋白水解产物可较长时间维持低水平SBP。

Different doses （low dose,0.0245 g/kg bw; medium dose,0.1225 g/kg bw; and high dose,0.6125 g/kg bw） of an α-casein hydrolysate prepared by sequential enzymatic hydrolysis with pepsin followed by trypsin were orally administered to spontaneously hypertensive rats （SHRs）,and the SHRs were tested for their systolic blood pressure （SBP） during 8 hours post-administration once a day for continuously 3 days in order to the anti-hypertension effect of single administration of theα-casein hydrolysate in SHRs. Moreover,in the continuous administration trial,high-dose administration was performed once at the time intervals of four hours twice a day and SBP measurement was done 2 hours after each administration. The results showed that in the single administration trial,normal Wistar rats administered high dose of theα-casein hydrolysate did not present any obvious change in SBP within 8 hours after administration. Each of the three doses had a notable inhibitory effect on the increase of SBP in SHRs. Among them,the antihypertensive effect of high dose was the best within the first 6 hours,and the SBP level of SHRs administered high dose for the longest period of time,reaching up to 6 hours,and the largest decrease by （29.50 ± 1.0） mmHg was observed at 5 hours. Furthermore,continuous administration of high dose could keep SBP at a lower level for a long time period.