黑灵芝多糖对小鼠脾淋巴细胞增殖及诱生细胞因子的影响

Effect of Polysaccharides from Ganoderma atrum on Spleen Lymphocyte Proliferation and Induction of Cytokine in Mice

目的:研究黑灵芝多糖(PSG-1)对小鼠脾淋巴细胞增殖及诱生细胞因子的影响。方法:采用四甲基偶氮唑盐(MTT)法检测PSG-1在不同质量浓度、不同时间对小鼠脾淋巴细胞增殖的影响,检测不同质量浓度PSG-1单独作用及其与丝裂原共同作用对小鼠T、B淋巴细胞增殖作用的影响;用酶联免疫吸附实验(ELISA)法测定白介素-2(IL-2)和肿瘤坏死因子-α(TNF-α)的含量。结果:PSG-1在20～160μg/mL质量浓度范围能显著促进脾细胞的增殖,且48h效果最佳;PSG-1能显著增强刀豆蛋白A(ConA)诱导的T淋巴细胞和脂多糖(LPS)诱导的B淋巴细胞的增殖作用;并且能显著诱导脾淋巴细胞分泌IL-2和TNF-α。结论:PSG-1能有效的促进脾淋巴细胞的增殖,并与ConA、LPS对T、B淋巴细胞的增殖有协同刺激作用,还可以通过IL-2和TNF-α发挥免疫增强作用。

Objective：To investigate the effect of polysaccharides from Ganoderma atrum （PSG-1） on spleen lymphocyte proliferation and induction of cytokine in mice. Methods：The effects of treatments with PSG-1 and with PSG-1 containing motigen at different concentrations on spleen lymphocyte proliferation were determined by MTT. The contents of IL-2 and TNF-α were determined by using ELISA. Results：PSG-1 at a concentration ranging from 20 to160 μg/mL resulted in a significant increase of lymphocyte proliferation,and 48 h administration gave the best proliferation. PSG-1 could also enhance the effect of Con A-induced T lymphocyte proliferation and the stimulation index of LPS-induced B lymphocytes. Moreover,PSG-1 could significantly increase the secretion of IL-2 and TNF-α in spleen lymphocyte. Conclusion：PSG-1 can effectively increase lymphocyte proliferation and enhance the effect of Con A,LPS-induced T and B cell growth. PSG-1 has the ability to enhance immune responses by increasing the secretion of IL-2 and TNF-α.