日本鬼鲉不同组织的8种同工酶谱

Distribution of eight enzymes in various organs of Inimicus japonicus

采用垂直聚丙烯酰胺平板电泳技术,对象山港野生日本鬼鲉(Inimicus japonicus)的9种组织器官的8种同工酶进行研究,并分析了其酶谱表型。结果表明,琥珀酸脱氢酶(Succinatedehydrogenase,简称SuDH)在所有组织中均存在,组织间差异较小;乳酸脱氢酶(Lactic dehydrogenase,简称LDH)、苹果酸脱氢酶((Malate dehydrogenase,简称MDH)、苹果酸酶(Malic enzyme,简称ME)、酯酶(Esterase,简称EST)、醇脱氢酶(Alcoholdehydrogenase,简称ADH)、过氧化物酶(Peroxidase,简称POD)、超氧化物歧化酶(Superoxide dismutase,简称SOD)则具有明显的组织特异性,且这些酶在表型、分布和活性上均表现出高度的组织特异性。

Eight isozymes in nine organs of Inimicus japonicus were studied by vertical polyacrylamide gel elec-trophoresis.Succinatedehydrogenase（SuDH） existed in all organs,showing little differences.Lactic dehydrogenase（LDH）,malate dehydrogenase（MDH）,malic enzyme（ME）,esterase（EST）,alcoholdehydrogenase（ADH）,peroxi-dase（POD）,and superoxide dismutase（SOD） showed clearly tissue-specific distributions.