微波辅助提取咖啡豆残渣中多酚

Microwave-assisted Extraction of Polyphenols from Coffee Bean Residue

从咖啡豆残渣中初步提取多酚。在有机溶剂提取多酚的基础上,采用微波辅助提取的方法,利用响应面分析,对提取条件进行研究。用Folin-Ciocalteus比色法测定多酚含量,以得率为评价指标,得到优化的提取参数:提取溶剂为50%(V/V)丙酮、微波功率296W、料液比1:20(g/mL)、微波提取时间17s。在此条件下,咖啡豆残渣提取多酚的得率为5.12%,是有机溶剂提取多酚得率(3.21%)的1.6倍,咖啡豆残渣提取的多酚得率大幅提高。本实验可为咖啡豆残渣中多酚的提取利用提供一定的参考。

In order to improve the utilization rate of coffee bean residue,polyphenols were extracted from coffee bean residue by organic solvent extraction with the aid of microwave. The optimal extraction conditions were explored by response surface methodology and the content of polyphenols was determined by Folin-Ciocalteus colorimetric method. The optimal parameters for the extraction of polyphenols were as follows：extraction solvent,50% （V/V） acetone; microwave power,296 W; material/liquid ratio,1：20; and extraction duration,17 s. The extraction rate of polyphenols was 5.12% under these optimal extraction conditions,1.6-fold higher than that obtained without the aid of microwave.