斑点叉尾鮰鱼骨脱脂及其制备CMC活性钙的工艺优化

Optimization of the Preparation of CMC Active Calcium from Degreased Ictalurus punctatus Bone

以斑点叉尾鮰鱼骨为实验材料,对脱脂鮰鱼骨粉制备工艺,脱脂骨粉在不同的温度、时间、柠檬酸和苹果酸的酸比例、酸质量浓度、骨粉粒度条件下转化成活性钙的效果进行研究。结果表明,鮰鱼骨脱脂的最佳工艺方案为5g/100mL NaOH溶液浸泡6h,脱脂后的鱼骨粉总钙含量为30.38%。实验采用柠檬酸和苹果酸活化脱脂鮰鱼骨粉制取CMC活性钙,其最佳工艺条件为柠檬酸和苹果酸的比例1:2、酸质量浓度15g/100mL、骨粉粒度120目、温度100℃、提取时间60min。此时,钙提取率可达87.13%。

The degreasing of Ictalurus punctatus bone and the preparation of CMC active calcium from degreased fish bone through thermal soaking in a mixed acid made up of citric acid and malic acid were studied in this work. The effects of soaking temperature and time,mixing ratio for malic acid and citric acid,total acid amount and fish bone granular size on calcium yield were examined. The optimal procedure for degreasing Ictalurus punctatus bone was soaking in 5% aqueous NaOH solution for 6 h,and the total calcium content after the degreasing was 30.38%. The optimal conditions for the preparation of CMC active calcium were determined to be：mixing ratio for malic acid and citric acid,1：2; total acid amount,15 g/100 mL; material granular size,120 mesh; soaking temperature,100 ℃; and soaking duration,60 min. A calcium yield of 87.13% was obtained under such conditions.