一株产阿魏酸酯酶青霉菌株的筛选、鉴定及生长特征

Isolation,Identification and Growth Characteristics of a Penicillium Strain Producing Feruloyl Esterases

从腐烂的木质纤维中筛选了一株产阿魏酸酯酶的菌株HDFE1,根据其形态特征、rDNAITS1-5.8S-ITS2序列及系统发育分析,鉴定菌株HDFE1为青霉属的橘青霉(Penicillium citrinum Thom)。菌株HDFE1最适生长温度为30°C,最适生长pH为6.0。该菌株在30°C、pH6.0、200r/min培养60h时,阿魏酸酯酶酶活力为最高,达20.75U/L。

The strain HDFE1,producing feruloyl esterases,was isolated from rotten wood fiber.It was identified as Penicillium citrinum Thom by study of morphology and rDNA ITS1-5.8S-ITS2 sequence and phylogenetic analysis.The optimum conditions for its growth were pH 6.0 and 30oC.At 30oC,pH 6.0,and 200 r/min for 60 h,the strain could produce feruloyl esterases at a yield 25 of 20.75 U/L.