微生物学实验模块“蛋白酶产生菌的筛选、培养和选育”的教改研究与实践

A Experiment Module of Isolation,Cultivation and Breeding of Protease-producing Bacteria for Microbiological Experiment Teaching

围绕"蛋白酶产生菌的筛选、培养和选育"主线,设计了一个由5个相互关联、前后衔接、形成一体的实验组成的综合性研究型实验模块,并进行教学实践。它们是:培养基的配制、分装与灭菌;产蛋白酶细菌的分离与纯化;产蛋白酶细菌的培养发酵与条件优化;产蛋白酶细菌的鉴定;产蛋白酶细菌的紫外诱变育种。

We integrated a group of related experiments focusing on isolation,cultivation and mutation breeding of protease-producing bacteria into an experiment module and applied it in microbiological experiment teaching.The experiment module included preparation and sterilization of media,isolation of protease-producing bacteria,optimization of cultivation conditions,characterization of the strain,mutation breeding of the strain by UV exposure.