动物源性大肠杆菌耐药性分析

Analysis of the Resistance to Antibiotics of the Animal Source Escherichia coli

目的:对吉林省长春市伊通地区分离出的113株动物源性大肠杆菌耐药性进行分析,了解猪源和鸡源大肠杆菌耐药程度及其耐药谱。方法:采用K-B纸片扩散法对大肠杆菌耐药性初步定性分析,并从中选出十株耐药谱不同的大肠杆菌测定其抗生素的最小抑菌浓度(MIC)。结果:113株均表现出不同程度的耐药,耐药谱广,且多表现为多重耐药。结论:动物源性大肠杆菌对抗生素的耐药性以多重耐药为主。113株对喹诺酮类、磺胺类、利福平耐药率达到90%以上,其中鸡源、猪源大肠杆菌耐药情况各不相同,二者均对丁胺卡那霉素、先锋必/舒巴坦较敏感,耐药率低于15%,可知丁胺卡那霉素、先锋必/舒巴坦可作为这些地区防治致病性大肠杆菌的首选药物。本实验结果对临床科学合理用药有重要指导意义。

Object： This article is to investigate the resistances of 113 animal source E. coli stains in the Changchun Yi tong region to the antibiotics, Understanding of Escherichia coli from pigs and chickens, drug resistance and drug resistance spectrum .Methods： Adopting the K-B slip method to the initial qualitative analysis, and to choose the 10 different resistance profile of E. coli determined the minimum inhibitory concentration of antibiotics （MIC） .Results： The 113 stains, having wide antibiotic resistance spectrum and multiple-antibiotic resistant, all display a different degree of resistance to the same medicine. Conclusions： At first the resistances of animal source E. coli are multiple-antibiotic-resistant. Secondly, the antibiotic resistance rate of all stains to quinolones, sulfadiazine and rifampicin are over 90%.Thirdly, the rate of chicken source is different to Swine Source＇s, but both of them are sensitive to amikacin and Sulbactam - Cefopcrazone, whose antibiotic resistance rate are 15%. The experimental results have an important guiding significance for clinical scientific and rational drug using.