豫北地区婴幼儿腹泻病毒病原学分析

Aetiology analysis of human epidemic gastroentertis virus in north district of Henan province

目的了解豫北地区婴幼儿腹泻标本中的轮状病毒、杯状病毒、腺病毒及诺如病毒4种病毒感染的情况。方法采集2009年1月至2009年12月新乡医学院一附院儿科收治的269例小儿腹泻患者粪便标本,采用酶联免疫吸附试验(ELISA)分别检测轮状病毒、杯状病毒、腺病毒、诺如病毒的抗原,并与50例健康儿童的标本相对照。对轮状病毒进行病毒株血清学检测,并采用逆转录聚合酶链式反应(RT-PCR)进行基因分型。结果患儿病毒病原检出率分别为轮状病毒59.2%、杯状病毒7.8%、腺病毒1.1%,未检出诺如病毒;其中混合感染率为2.2%。同时用RT-PCR及ELISA2种方法检查56份粪便标本中轮状病毒,检查一致率达96.43%。56株轮状病毒G血清型分型:血清Ⅰ型3份,Ⅱ型3份,Ⅲ型43份,Ⅸ型4份,未能分型3份。对8株轮状病毒P分型,其中7株为G3P8,1株为G9P8。轮状病毒感染具有明显季节性,以秋、冬季多,杯状病毒以秋季多。结论豫北地区婴幼儿腹泻的病毒病原中以轮状病毒为主,杯状病毒次之,腺病毒检出率低。流行的轮状病毒以G3型为主,G3P8多见。

Objective To investigate the infection condition of rotavirus,calicivirus,adenovirus and norovirus among infantile diarrhea specimens in north district of Henan province. Methods Fecal specimens were collected from 269 diarrhea infants hospitalized in the first affiliated hospital of Xinxiang medical college from January to December in 2009. Enzyme linked im-munosorbent assay （ ELISA） were performed to detect the antigens of rotavirus,calicivirus,adenovirus,and norovirus,and then compared with the specimens of 50 healthy infants. The orrhology testing was taken to detected the rotavirus,and reverse transcription and polymerase chain reaction（ RT-PCR） was adopted to type the gene. Results The detection rates of rotavirus,calicivirus and adenovirus were 59. 2% ,7. 8% and 1. 1% respectively,norovirus was not detected. The combined infection rate was 2. 2%. Fifty-six fecal specimens were tested by ELISA and RT-PCR,and the results showed that the two methods got the consistency of 96. 43%. Fifty-six rotaviruses of serotype G were further typed as serotype ？？？ cases） ,？？？ cases ） ,？？？3 cases） and ？？cases） ,with 3 cases untyped. Among the 8 rotaviruses of type P,7 were type as G3P8 and one G9P8. The rotavirus infection showed significantly seasonality,with a high prevalence in fall and winter. While the prevalence of calicivirus was high in fall. Conclusion Rotavirus is the major viral pathogen accounting for infantile diarrhea in north district of Henan province,followed by calicivirus and adenovirus. The type of epidemic rotavirus is mainly G3,especially G3P8.