野生苎麻微生物脱胶优势菌的选育及其应用

Screening and applications of superior strain for biological retting of wild ramie

为实现对野生苎麻的生物脱胶,采用平板稀释法从沤麻液中分离得到37株菌株,并采用平板透明圈法和DNS酶活测定法筛选野生苎麻生物脱胶用优势菌株。实验结果表明,TJ03菌株的透明圈与酶活值均为最大,其水解透明圈与菌落面积的比值为19.98,果胶酶酶活和甘露聚糖酶酶活分别为9.54、8.63 U/mL。最后对TJ03的脱胶条件进行了研究,得到其最佳脱胶条件为:初始pH值7.5,浴比1∶25,接种量15%,发酵时间4 d,发酵温度35℃。在上述条件下,野生苎麻生物脱胶后残胶率为14.09%,实现了纤维从韧皮中分离。

In order to achieve bio-degumming of the wild ramie,37 strains were isolated from retting water of the wild ramie by agar plate dilution method,and superior stain was screened by transparent circle in combination with DNS method.It was found that transparent circle and enzymatic activity of TJ03 strain were the best.The ratio of transparent circle area and colony area was 19.98,pectinase activity and mannanase activity were 9.54,8.63 U /mL,respectively.Then microbial strain TJ03 was used for degumming of the wild ramie.The optimal conditions of retting wild ramie were pH 7.5,temperature 35 ℃,bath ratio 1∶ 25 and 15% inoculum size of strain TJ03,with residual gum content of wild ramie only 14.09%.Fiber bast was separated from the natural gum and woody matter of the stalk of wild ramie.