清热利湿中药对梗阻性黄疸大鼠肝PKC及TNF-R1表达的影响

Effect of Clearing Heat and Removing Dampness Herbs on Expression of PKC and TNF-R1 of Liver in Rats with Obstructive Jaundice

目的:观察清热利湿中药对梗阻性黄疸大鼠肝PKC及TNF-R1表达的影响。方法:制作梗阻性黄疸大鼠模型,分为中药组、生理盐水组、模型组和假手术组,分别于3、7、14、21天处死大鼠,取肝组织标本固定、包埋、染色,采用免疫组织化学SABC法检测PKC、TNF-R1的表达。结果:随着时间的变化,盐水组与中药组PKC蛋白及TNF-R1蛋白的表达逐渐升高、到14天达高峰,而中药组PKC蛋白及TNF-R1蛋白的表达较盐水组明显偏低,且有显著性差异(P<0.05)。结论:清热利湿中药对PKC及TNF-R1蛋白表达有一定的抑制作用,可对抗肝细胞凋亡,保护肝功能。

Objective：observe the expressive effect of damp-heat removing herbs on obstructive jaundice rats liver PKC and TNF-R1.Method：make obstructive jaundice rat model,divide them into herb group and normal brine group then execute them in third,seventh,fourteenth and twenty-first day respectively;take their liver tissues to fix,embed,dye and adopting immunity histochemistry SABC to examine PKC,TNF-R1＇s expression.Result：with timepassing,the expression of PKC albumen and TNF-R1 albumen in herb group and brine group will gradually increase to the peak in 14th day.However,the expression of PKC albumen and TNF-R1 albumen in herb group is obviously lower than brine group,which has salient discrepancy in statistics（P〈0.05）.Conclusion：damp-heat removing herbs will have definite inhibition effect on PKC and TNF-R1 albumen＇s expression,therefore make opposed liver cell wither and protect liver function.