昆明西山野生蘡薁葡萄资源及其遗传多样性分析

A study on the distribution and genetic diversities of wild grape resources Vitis bryoniaegolia in Xishan mountain,Kunming

调查了昆明西山野生蘡薁葡萄(Vitis bryoniaegolia Bge)的分布特征,并利用RAPD分子标记对其5个群体的遗传多样性进行了分析.采用5个RAPD引物扩增,共产生71条DNA条带,其中多态条带68条.在物种水平上,蘡薁葡萄多态位点百分率(PPB)为95.77%,Nei's基因多样性指数(H)为0.3442,Shannon's多样性信息指数(Hsp)为0.5100;在群体水平上,PPB差异较大(35.21%～84.51%),平均值为60.85%,H平均为0.2234,Ho平均为0.3305.各群体间的Nei's遗传一致度(I)范围为0.7171～0.9489.基于Nei's遗传多样性分析得出的群体间遗传分化系数Gst=0.3269,表明西山蘡薁葡萄的遗传多样性主要来源与群体内而不是群体间.Mantel检测发现,群体间的遗传距离和物理距离之间呈显著的正相关(r=0.981,P=0.019<0.05).

The ecological distribution characters of Vitis bryoniaeglia Beg from Xishan mountain in Kunming were investigated,the genetic diversities based on the RAPD markers were studied in 5 groups of Vitis bryoniaegolia Bge.The results indicated that 68 diversified loci in total 71 DNA bands were amplified by using 5 random primers.At species level,the percentage of polymorphic bands （PPB）,Nei＇s gene diversity （H） and the Shannon＇s information index （Hsp） were 95.77%,0.344 2 and 0.510 0,respectively.While at groups level,the average PPB was 60.85 % （varied from 35.21 % —84.51%）.The average H and Ho were 0.223 4 and 0.330 5,respectively.The coefficient of genes differentiation among groups （Gst） based on Nei＇s genetic distance was 0.326 9,which implied that the gene variation was originated mainly within the groups （77.31%） other than among （32.69%） the groups.Genetic identity （I） of the 5 groups was varied from 0.717 1 to 0.948 9.The geographical distance was significant correlated with the genetic distance based by the Mantel analysis as r=0.981,P=0.019 0.05.