摘要
目的:研究三七三醇皂苷(PTS)对局灶性脑缺血再灌注后脑室下区(SVZ)细胞增殖及星形胶质细胞的影响。方法:60只雄性SD大鼠随机分为假手术、生理盐水(NS)和PTS组,每组设再灌注3、7、14、21d亚组,每个时段各5只。采用改良的线栓法制备大脑右侧中动脉阻塞2h的大鼠短暂局灶性脑缺血模型,观察5-溴脱氧尿嘧啶和胶质纤维酸性蛋白(GFAP)在SVZ的表达情况。结果:再灌注后7d,PTS组与NS组SVZ细胞增殖水平达到峰值,至再灌注21d细胞增殖水平已明显回落,但仍高于正常水平;再灌注后7、14d,PTS组SVZ细胞增殖水平显著高于NS组。再灌注后3d,PTS组与NS组SVZ细胞GFAP表达达到峰值,至再灌注14d已恢复正常水平;再灌注后3、7d,PTS组SVZ的GFAP阳性细胞数显著高于NS组。结论:PTS可促进缺血再灌注后SVZ细胞的增殖水平,并上调GFAP的表达。
OBJECTIVE:To explore the effect of PTS on cell proliferation and astrocyte of sbuventricular zone(SVZ)in rats with focal ischemia reperfusion.METHODS:60 male SD rats were randomly divided into sham operation group,NS group and PTS group.Each group was subdivided into 3 d reperfusion subgroup,7 d reperfusion subgroup,14 d reperfusion subgroup and 21d reperfusion subgroup with each group of 5 rats.The model of focal cerebral ischemia in rat was established in right middle cerebral artery occlusion(MCAO)by modified suture.The expressions of 5-bromodeoxyuridine(Brdu)and GFAP in SVZ were observed.RESULTS:The level of cell proliferation in PTS group and NS group peaked at 7 d after MCAO reperfusion,and decreased obviously but higher than normal levels at 21 d.Compared with NS group,the levels of cell proliferation in the PTS group were up-regulated at 7 d and 14 d after reperfusion.The expression of GFAP in PTS group and NS group peaked at 3 d after reperfusion and returned to normal at 14 d.The positive GFAP cells in PTS group was more than in NS group.CONCLUSION:PTS could enhance the level of cell proliferation and the expression of GFAP in SVZ in adult rats with focal cerebral ischemia-reperfusion.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第43期4061-4063,共3页
China Pharmacy