三七三醇皂苷对脑缺血大鼠脑室下区细胞增殖及星形胶质细胞的影响

Effect of PTS on Cell Proliferation and Astrocyte of Subventricular Zone in Rats with Cerebral Ischemia

目的:研究三七三醇皂苷(PTS)对局灶性脑缺血再灌注后脑室下区(SVZ)细胞增殖及星形胶质细胞的影响。方法:60只雄性SD大鼠随机分为假手术、生理盐水(NS)和PTS组,每组设再灌注3、7、14、21d亚组,每个时段各5只。采用改良的线栓法制备大脑右侧中动脉阻塞2h的大鼠短暂局灶性脑缺血模型,观察5-溴脱氧尿嘧啶和胶质纤维酸性蛋白(GFAP)在SVZ的表达情况。结果:再灌注后7d,PTS组与NS组SVZ细胞增殖水平达到峰值,至再灌注21d细胞增殖水平已明显回落,但仍高于正常水平;再灌注后7、14d,PTS组SVZ细胞增殖水平显著高于NS组。再灌注后3d,PTS组与NS组SVZ细胞GFAP表达达到峰值,至再灌注14d已恢复正常水平;再灌注后3、7d,PTS组SVZ的GFAP阳性细胞数显著高于NS组。结论:PTS可促进缺血再灌注后SVZ细胞的增殖水平,并上调GFAP的表达。

OBJECTIVE：To explore the effect of PTS on cell proliferation and astrocyte of sbuventricular zone（SVZ）in rats with focal ischemia reperfusion.METHODS：60 male SD rats were randomly divided into sham operation group,NS group and PTS group.Each group was subdivided into 3 d reperfusion subgroup,7 d reperfusion subgroup,14 d reperfusion subgroup and 21d reperfusion subgroup with each group of 5 rats.The model of focal cerebral ischemia in rat was established in right middle cerebral artery occlusion（MCAO）by modified suture.The expressions of 5-bromodeoxyuridine（Brdu）and GFAP in SVZ were observed.RESULTS：The level of cell proliferation in PTS group and NS group peaked at 7 d after MCAO reperfusion,and decreased obviously but higher than normal levels at 21 d.Compared with NS group,the levels of cell proliferation in the PTS group were up-regulated at 7 d and 14 d after reperfusion.The expression of GFAP in PTS group and NS group peaked at 3 d after reperfusion and returned to normal at 14 d.The positive GFAP cells in PTS group was more than in NS group.CONCLUSION：PTS could enhance the level of cell proliferation and the expression of GFAP in SVZ in adult rats with focal cerebral ischemia-reperfusion.